Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis.
Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis.
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DOI：
10.1128/aac.00898-18
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发表时间：
2018-11
影响因子：
4.9
通讯作者：
Williamson DA
中科院分区：
文献类型：
--
作者：
Carter GP;Ussher JE;Da Silva AG;Baines SL;Heffernan H;Riley TV;Broadbent R;van der Linden A;Lee J;Monk IR;Stinear TP;Howden BP;Williamson DA
凝固酶阴性葡萄球菌（con），如头型葡萄球菌，是新生儿重症监护病房（NICUs）血液感染的主要原因。近年来，一种独特的猪头链球菌克隆（命名为猪头链球菌NRCS-A）已成为国际上新生儿重症监护病房的重要病原体。凝固酶阴性葡萄球菌（con），如头型葡萄球菌，是新生儿重症监护病房（NICUs）血液感染的主要原因。近年来，一种独特的猪头链球菌克隆（命名为猪头链球菌NRCS-A）已成为国际上新生儿重症监护病房的重要病原体。在这里，来自新西兰（NZ）的122株猪链球菌进行了全基因组测序（WGS），这些数据与公开的猪链球菌序列读数进行了补充。进行了系统发育和比较基因组分析，并对代表性分离株进行了抗菌素耐药性、生物膜形成和质粒分离稳定性的表型评估。在新西兰发现了一个与新生儿和新生儿重症监护病房环境相关的独特的猪头链球菌谱系。该谱系的分离株产生更高水平的生物膜，对氯己定表现出更高水平的耐受性，并具有多药耐药。虽然在核心基因组水平上与全球流行的NICU相关的us - capitis菌株相似，但新西兰NICU us - capitis分离株携带一种新的稳定维持的多药耐药质粒，而非NICU分离株中不存在这种质粒。新生儿血培养分离株与在听诊器和新生儿保育箱等环境中发现的头链球菌分离株难以区分，但通常与新生儿重症监护病房工作人员携带的分离株不同。这项研究表明，新生儿重症监护室环境是由猪链球菌引起的新生儿败血症的潜在储库，并强调了基于基因组学的跟踪和监测的能力，为未来医院感染控制实践提供信息，旨在遏制这一重要新生儿病原体的传播。
Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 122 S. capitis isolates from New Zealand (NZ) underwent whole-genome sequencing (WGS), and these data were supplemented with publicly available S. capitis sequence reads. Phylogenetic and comparative genomic analyses were performed, as were phenotypic assessments of antimicrobial resistance, biofilm formation, and plasmid segregational stability on representative isolates. A distinct lineage of S. capitis was identified in NZ associated with neonates and the NICU environment. Isolates from this lineage produced increased levels of biofilm, displayed higher levels of tolerance to chlorhexidine, and were multidrug resistant. Although similar to globally circulating NICU-associated S. capitis strains at a core-genome level, NZ NICU S. capitis isolates carried a novel stably maintained multidrug-resistant plasmid that was not present in non-NICU isolates. Neonatal blood culture isolates were indistinguishable from environmental S. capitis isolates found on fomites, such as stethoscopes and neonatal incubators, but were generally distinct from those isolates carried by NICU staff. This work implicates the NICU environment as a potential reservoir for neonatal sepsis caused by S. capitis and highlights the capacity of genomics-based tracking and surveillance to inform future hospital infection control practices aimed at containing the spread of this important neonatal pathogen.