A characterization of the erasure phenomenon in dictyostelium.
A characterization of the erasure phenomenon in dictyostelium.
复制标题
盘基网柄菌擦除现象的表征。
DOI：
10.1016/0012-1606(77)90111-7
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发表时间：
1977
影响因子：
2.7
通讯作者：
D. Soll
中科院分区：
文献类型：
--
作者：
D. Waddell;D. Soll
当发育中的网骨藻培养物被分解时，变形虫能够在大约原来时间的十分之一的时间内重演它们先前所经历的形态发生阶段。当发育中的培养物被分解并置于生长培养基中时，它们在一个离散的步骤中失去了这种快速重演的能力，我们以前称之为“擦除”。在这份报告中，擦除现象的几个特点进行了分析，并提出了数据表明：（1）擦除的时间和营养需求的变化，通过发展的细胞进展;（2）的能力，快速重演的不同阶段可以在不同的时间被擦除，表明事件的影响重演的不同阶段的时间是可分离的。对于来自形态发生的紧密聚集体阶段的细胞，我们提供的数据表明：（1）擦除依赖于一个从头蛋白质合成的时期，该时期从细胞接种到营养培养基后30分钟开始，并延伸到擦除的时刻;（2）擦除事件的稳定期发生在擦除后50分钟内，并依赖于从头蛋白质合成;（3）转录抑制剂对聚集后期的擦除时间无显著影响，但对聚集前期的擦除有延迟作用;以及（4）擦除发生在细胞生长重新开始后约2小时，在第一次同步细胞分裂完成前数小时。营养培养基根据这些观察的复杂性和可能的性质的能力，迅速概括和擦除现象进行了讨论。
When developing Dictyostelium cultures are disaggregated, the amoebas are capable of recapitulating the morphogenetic stages they had previously progressed through in approximately 1 10 th the original time. When developing cultures are disaggregated and placed in growth medium, they lose this capacity for rapid recapitulation in a discrete step which we previously called “erasure.” In this report, several characteristics of the erasure phenomenon are analyzed, and data are presented which demonstrate that:(1) the timing and nutrient requirements of erasure change as cells progress through development; and (2) the capacity for the rapid recapitulation of different stages can be erased at different times, indicating that events which influence the recapitulation timing of different stages are dissociable. For cells derived specifically from the tight aggregate stage of morphogenesis, we have presented data which indicate that:(1) erasure depends upon a period of de novo protein synthesis which begins 30 min after cells are inoculated into nutrient medium and which extends to the moment of erasure;(2) a period of stabilization for the erasure event (s) occurs within 50 min after erasure and depends upon de novo protein synthesis;(3) inhibitors of transcription do not significantly affect the erasure time of later aggregation stages, but retard the erasure of an earlier stage; and (4) erasure occurs approximately 2 hr after the reinitiation of cell growth and several hours prior to the first synchronous cell division in complete nutrient medium. The complexity and possible nature of both the capacity to recapitulate rapidly and the erasure phenomenon are discussed in light of these observations.