Fresh organically grown ginger (Zingiber officinale):: composition and effects on LPS-induced PGE2 production
Fresh organically grown ginger (Zingiber officinale):: composition and effects on LPS-induced PGE2 production
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DOI：
10.1016/j.phytochem.2004.06.008
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发表时间：
2004-07-01
期刊：
影响因子：
3.8
通讯作者：
Timmermann, BN
中科院分区：
文献类型：
--
作者：
Jolad, SD;Lantz, RC;Timmermann, BN
采用气相色谱与质谱法相结合的方法，首次成功地分析了生姜经三甲基硅衍生物修饰后的非挥发性刺激性成分，并成功地分析了有机栽培的中国鲜姜和日本黄姜的二氯甲烷提取物中未修饰的部分纯化部分。这一分析结果检测到了20种迄今未知的天然产物和31种以前报告的生姜成分。这些化合物包括多酚、二氢双酚、姜酚、姜酚的乙酰衍生物、姜辣素、3-二氢姜酚、姜二醇、姜二醇的单乙酰和双乙酰衍生物、1-脱氢姜二酮、二芳基庚烷类化合物以及其中一些化合物的甲醚衍生物。研究了姜辣素的热降解为姜黄酮、姜辣素及相关化合物。这两个品种的主要成分是[6]-姜酚，这是药用泽兰的化学标志。对所有化合物的质谱图进行了描述和解释。用体外前列腺素E(2)法检测了硅胶层析物的抗炎活性。大部分含有姜辣素和/或姜辣素衍生物的部位对脂多糖诱导的前列腺素E(2)的产生有很好的抑制作用。(C)2004爱思唯尔有限公司。保留所有权利。
Gas chromatography in conjunction with mass spectrometry, a technique previously employed to analyze non-volatile pungent components of ginger extracts modified to trimethylsilyl derivatives, was applied successfully for the first time to analyze unmodified partially purified fractions from the dichloromethane extracts of organically grown samples of fresh Chinese white and Japanese yellow varieties of ginger, Zingiber officinale Roscoe (Zingiberaceae). This analysis resulted in the detection of 20 hitherto unknown natural products and 31 compounds previously reported as ginger constituents. These include paradols, dihydroparadols, gingerols, acetyl derivatives of gingerols, shogaols, 3-dihydroshogaols, gingerdiols, mono- and diacetyl derivatives of gingerdiols, 1-dehydrogingerdiones, diarylheptanoids, and methyl ether derivatives of some of these compounds. The thermal degradation of gingerols to gingerone, shogaols, and related compounds was demonstrated. The major constituent in the two varieties was [6]-gingerol, a chemical marker for Z. officinale. Mass spectral fragmentation patterns for all the compounds are described and interpreted. Anti-inflammatory activities of silica gel chromatography fractions were tested using an in vitro PGE(2) assay. Most of the fractions containing gingerols and/or gingerol derivatives showed excellent inhibition of LPS-induced PGE(2) production. (C) 2004 Elsevier Ltd. All rights reserved.