POLARIZED INTEGRIN MEDIATES HUMAN KERATINOCYTE ADHESION TO BASAL LAMINA
POLARIZED INTEGRIN MEDIATES HUMAN KERATINOCYTE ADHESION TO BASAL LAMINA
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DOI：
10.1073/pnas.87.17.6888
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发表时间：
1990-09-01
影响因子：
11.1
通讯作者：
QUARANTA, V
中科院分区：
文献类型：
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作者：
DELUCA, M;TAMURA, RN;QUARANTA, V
上皮细胞与基质的相互作用对组织结构至关重要。间接免疫荧光和免疫沉淀法表明，角质形成细胞表达的整合素主要为αEβ4(又称α6β4)和α2β1。αEβ4整合素定位于与基底膜接触的基底层细胞表面，而α2β1/α3β1整合素不存在于基底面，仅定位于基底层和棘层角质形成细胞的侧面。抗β4抗体有效地抑制角质形成细胞与基质细胞或纯化的层粘连蛋白的黏附，而抗β1抗体无效。只有抗β4抗体能够分离已建立的角质形成细胞集落。这些数据表明，αEβ4介导角质形成细胞与基底膜的黏附，而β1亚家族参与角质形成细胞的细胞间黏附。
Epithelial cell interactions with matrices are critical to tissue organization. Indirect immunofluorescence and immunoprecipitations of cell lysates prepared from stratified cultures of human epidermal cells showed that the major integrins expressed by keratinocytes are alpha E beta 4 (also called alpha 6 beta 4) and alpha 2 beta 1. The alpha E beta 4 integrin is localized at the surface of basal cells in contact with the basement membrane, whereas alpha 2 beta 1/alpha 3 beta 1 integrins are absent from the basal surface and are localized only on the lateral surface of basal and spinous keratinocytes. Anti-beta 4 antibodies potently inhibited keratinocyte adhesion to matrigel or purified laminin, whereas anti-beta 1 antibodies were ineffective. Only anti-beta 4 antibodies were able to detach established keratinocyte colonies. These data suggest that alpha E beta 4 mediates keratinocyte adhesion to basal lamina, whereas the beta 1 subfamily is involved in cell-cell adhesion of keratinocytes.