The role of chemical sputtering during plasma sterilization of Bacillus atrophaeus
The role of chemical sputtering during plasma sterilization of Bacillus atrophaeus
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DOI：
10.1088/0022-3727/40/9/024
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发表时间：
2007-05-07
影响因子：
3.4
通讯作者：
von Keudell, A.
中科院分区：
文献类型：
--
作者：
Opretzka, J.;Benedikt, J.;von Keudell, A.
通过等离子体放电灭活细菌具有处理时间短、对被灭菌物体的损害最小以及危险化学品使用最少的独特优点。等离子体从任何给定的前体气体产生离子、原子和UV光子的反应通量，并且预期是用于此类灭菌应用的可行方法。然而，有害生物系统的基于等离子体的灭活尚未被广泛使用，因为任何验证都受到关于等离子体和生物系统之间界面处的相互作用机制的有限知识的阻碍。通过使用氢原子、氩离子和UV光子的量化束，以非常受控的方式模拟了在典型的氩-氢等离子体中对细菌的处理。作为一个例子，研究了萎缩芽孢杆菌内生孢子的灭活。它表明，H原子单独的影响不会导致细菌失活。相反，原子和低能离子的同时撞击导致内孢壳穿孔。在等离子体处理过程中发生了相同的过程，并解释了细菌的有效灭活。
The inactivation of bacteria by plasma discharges offers the unique benefits of short treatment times, minimal damage to the objects being sterilized and minimal use of hazardous chemicals. Plasmas produce reactive fluxes of ions, atoms and UV photons from any given precursor gas and are expected to be a viable method for such sterilization applications. The plasma based inactivation of harmful biological systems is, however, not yet widely used, because any validation is hampered by the limited knowledge about the interaction mechanisms at the interface between a plasma and a biological system. By using quantified beams of hydrogen atoms, argon ions and UV photons, the treatment of bacteria in a typical argon - hydrogen plasma is mimicked in a very controlled manner. As an example the inactivation of endospores of Bacillus atrophaeus is studied. It is shown that the impact of H atoms alone causes no inactivation of bacteria. Instead, the simultaneous impact of atoms and low energy ions causes a perforation of the endosporic shell. The same process occurs during plasma treatment and explains the efficient inactivation of bacteria.