Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin
Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin
复制标题
一氧化氮和赤霉素预浸种诱导小麦发芽过程中的耐冷性
DOI：
10.1007/s10725-013-9805-8
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发表时间：
2013-09-01
影响因子：
4.2
通讯作者：
Jiang, Dong
中科院分区：
文献类型：
--
作者：
Li, Xiangnan;Jiang, Haidong;Jiang, Dong
低温抑制了晚播冬小麦种子萌发和幼苗建成，是影响晚播冬小麦产量形成的主要因素之一。冬小麦（Triticum aestivumL.）分别用硝普钠（SNP）和赤霉素（GA 3）预处理后，在低温条件下萌发。SNP和GA 3预处理提高了种子的发芽率、发芽指数、胚芽鞘和胚根的重量和长度，降低了低温下种子的平均发芽时间和重量。外源NO和GA 3增加了种子呼吸速率，沿着淀粉酶活性的增加，促进了淀粉的降解。此外，有效的抗氧化系统被NO激活，并有效地降低丙二醛和过氧化氢（H2O2）的浓度。外源NO和GA3也促进了幼苗的生长，提高了种子萌发率，并在低温下维持了幼苗生长过程中更好的活性氧平衡。说明在小麦种子萌发和成苗过程中，外源NO比GA3更能有效缓解低温胁迫。
Chilling depresses seed germination and seedling establishment, and is one major constraint to grain yield formation in late sown winter wheat. Seeds of winter wheat (Triticum aestivumL.) were separately pre-soaked with sodium nitroprusside (SNP, as nitric oxide donor) and Gibberellic acid (GA3) before germination and then germinated under low temperature. SNP and GA3pre-treatment increased seed germination rate, germination index, weights and lengths of coleoptile and radicle, while they decreased mean germination time and weight of seeds germinating under low temperature. Exogenous NO and GA3increased seed respiration rate and promoted starch degradation along with increased amylase activities. In addition, efficient antioxidant systems were activated by NO, and which effectively reduced concentrations of malondialdehyde and hydrogen peroxide (H2O2). Seedling growth was also enhanced by exogenous NO and GA3as a result of improved seed germination and maintenance of better reactive oxygen species homeostasis in seedling growing under chilling temperatures. It is indicated that exogenous NO was more effective than GA3in alleviating chilling stress during seed germination and seedling establishment in wheat.