NEURAL STEM-CELLS IN THE ADULT MAMMALIAN FOREBRAIN - A RELATIVELY QUIESCENT SUBPOPULATION OF SUBEPENDYMAL CELLS
NEURAL STEM-CELLS IN THE ADULT MAMMALIAN FOREBRAIN - A RELATIVELY QUIESCENT SUBPOPULATION OF SUBEPENDYMAL CELLS
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DOI：
10.1016/0896-6273(94)90046-9
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发表时间：
1994-11-01
期刊：
影响因子：
16.2
通讯作者：
VANDERKOOY, D
中科院分区：
文献类型：
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作者：
MORSHEAD, CM;REYNOLDS, BA;VANDERKOOY, D
从成年小鼠前脑的侧脑室解剖室管膜下对于体外形成克隆衍生的细胞球是必要的和足够的，所述细胞球表现出干细胞特性，例如自我维持和产生大量的后代，所述后代包括在中枢神经系统中发现的主要细胞类型。杀死在体内的室管膜下的组成性增殖细胞对在体外分离的干细胞的数量没有影响，并且通过在室管膜下发现的相对静止的干细胞在体内诱导室管膜下的完全再增殖。耗尽相对静止的细胞群体内的室管膜下在体内的结果在相应的减少在体外形成的球体和在体内的组成性增殖细胞的最终数量，这表明一个相对静止的室管膜下细胞是在体内的神经干细胞的来源。
Dissection of the subependyma from the lateral ventricle of the adult mouse forebrain is necessary and sufficient for the in vitro formation of clonally derived spheres of cells that exhibit stem cell properties such as self-maintenance and the generation of a large number of progeny comprising the major cell types found in the central nervous system. Killing the constitutively proliferating cells of the subependyma in vivo has no effect on the number of stem cells isolated in vitro and induces a complete repopulation of the subependyma in vivo by relatively quiescent stem cells found within the subependyma. Depleting the relatively quiescent cell population within the subependyma in vivo results in a corresponding decrease in spheres formed in vitro and in the final number of constitutively proliferating cells in vivo, suggesting that a relatively quiescent subependymal cell is the in vivo source of neural stem cells.