Assessing the Role of Melatonin in the Modulation of Visual Functions in the Mouse.
Assessing the Role of Melatonin in the Modulation of Visual Functions in the Mouse.
复制标题
评估褪黑激素在小鼠视觉功能调节中的作用。
DOI：
10.1007/978-1-0716-2593-4_38
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tosini,Gianluca
中科院分区：
文献类型：
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作者：
Baba,Kenkichi;Tosini,Gianluca
视网膜电图（ERG）是一种评估视网膜功能的无创方法。可用于诊断各种视网膜病变，如光感受器营养不良、糖尿病视网膜病变、黄斑变性、青光眼等。在从鱼类到人类的动物研究中，ERG也是评估视网膜功能的可靠工具。褪黑激素是一种调节视网膜内几种视网膜功能的神经激素，先前的研究表明，褪黑激素在人类和其他脊椎动物的ERG调节中起重要作用。本章描述了利用ERG评估小鼠视网膜功能的实验方法，以及如何评估褪黑素的贡献。介绍了ERG测量所需的材料、环境设置、记录程序和分析。
The electroretinogram (ERG) is a noninvasive method to evaluate retinal function. It can be applied to patients to be diagnosed a variety of retinal pathologies such as photoreceptor dystrophy, diabetic retinopathy, macular degeneration, and glaucoma. ERG has also been a reliable tool to assess retinal functions in animal studies that range from fish to humans. Melatonin is a neurohormone that regulates several retinal functions within the retina, and previous studies have shown that melatonin plays an important role in the modulation of the ERG in humans and other vertebrates. This chapter describes experimental methods to evaluate retinal function using ERG in the mouse and how to assess the contribution of melatonin. An introduction is provided for materials, environmental settings, recording procedures, and analysis necessary for ERG measurements.