A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometry
A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometry
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DOI：
10.1002/pmic.200600756
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发表时间：
2007-02-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Jing
中科院分区：
文献类型：
--
作者：
Pan, Sheng;Zhu, David;Zhang, Jing
人脑脊液（CSF）是研究聚集性神经退行性疾病蛋白生物标志物的重要来源。在确定每种疾病特有的生物标志物之前，有必要对脑脊液蛋白进行系统和广泛的分类。然而，由于脑脊液翻译后修饰的巨大复杂性、蛋白质浓度的巨大动态范围和巨大的蛋白质异质性，对现有的蛋白质组学技术提出了重大挑战，无法对人类脑脊液蛋白质组进行深入、无偏见的分析。为了克服这些困难，在过去的几年中，我们利用了几种不同的分离方法和质谱平台，极大地提高了脑脊液蛋白质谱的鉴定范围和深度，以表征脑脊液蛋白质组。使用严格的蛋白质组学标准，在特征良好的汇集的人脑脊液样本中共鉴定出2594种蛋白质。本报告总结了迄今为止我们在全面表征人类脑脊液蛋白质组方面所做的努力。
Human cerebrospinal fluid (CSF) is an important source for studying protein biomarkers of agerelated neurodegenerative diseases. Before characterizing biomarkers unique to each disease, it is necessary to categorize CSF proteins systematically and extensively. However, the enormous complexity, great dynamic range of protein concentrations, and tremendous protein heterogeneity due to post-translational modification of CSF create significant challenges to the existing proteomics technologies for an in-depth, nonbiased profiling of the human CSF proteome. To circumvent these difficulties, in the last few years, we have utilized several different separation methodologies and mass spectrometric platforms that greatly enhanced the identification coverage and the depth of protein profiling of CSF to characterize CSF proteome. In total, 2594 proteins were identified in well-characterized pooled human CSF samples using stringent proteomics criteria. This report summarizes our efforts to comprehensively characterize the human CSF proteome to date.