Xenopus oocyte cAMP‐dependent protein kinases before and during progesterone‐induced maturation
Xenopus oocyte cAMP‐dependent protein kinases before and during progesterone‐induced maturation
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黄体酮诱导成熟之前和过程中非洲爪蟾卵母细胞 cAMP 依赖性蛋白激酶
DOI：
10.1002/jez.1402070304
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发表时间：
1979
影响因子：
--
通讯作者：
L.Dennis Smith
中科院分区：
文献类型：
--
作者：
C. M. O’Connor;L.Dennis Smith
两个cAMP依赖性激酶的活动，对应于I型和II型同工酶的特点，在其他组织中，已部分纯化非洲爪蟾卵母细胞之前和期间，胆甾酮诱导的体外成熟。这两种活性在卵巢卵母细胞中以大约等摩尔浓度存在，总激酶浓度估计最低为0.2-0.4 μM。两种同工酶的相对活性在卵母细胞成熟过程中没有变化。
Two cAMP-dependent kinase activities, corresponding to the Type I and Type II isozymes characterized in other tissues, have been partially purified from Xenopus laevis oocytes both before and during progesteroneinduced maturation in vitro. The two activities are present in approximately equimolar concentrations in ovarian oocytes and the total kinase concentration has been estimated at 0.2–0.4 μM minimally. The relative activities of the two isozymes do not change during oocyte maturation.