Optimization of medium composition for production of antifungal active substance from Streptomyces hygroscopicus BS-112
Optimization of medium composition for production of antifungal active substance from Streptomyces hygroscopicus BS-112
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DOI：
10.5897/ajmr11.928
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发表时间：
2012-01
影响因子：
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通讯作者：
N. Zhang;Z. Qi;Huiguo Duan;Yuhua Xie;Jian Yu;Changxu Lu;Xunli Liu
中科院分区：
文献类型：
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作者：
N. Zhang;Z. Qi;Huiguo Duan;Yuhua Xie;Jian Yu;Changxu Lu;Xunli Liu
吸水链霉菌BS-112是从山东泰山林地中分离到的一株链霉菌。该菌具有广谱抗真菌和抗菌活性。筛选了产生抗真菌活性物质所必需的培养基。采用Plackett-Burman设计（PBD）和响应面法（RSM）对吸水性芽孢杆菌BS-112的发酵条件进行优化。最初，使用PBD测试了葡萄糖、大豆粉和KH 2 PO 4，发现它们是影响活性物质产生的重要因素。然后使用最陡上升法来确定培养基中包含的每个因子的最佳区域。采用中心组合设计（CCD）对各重要因素的浓度进行优化。结果表明，最佳培养基组成（g/l）为：玉米粉5.00、葡萄糖18.61、黄豆粉31.15、KH_2PO_40.38、MgSO_40.85。与使用基础培养基生产的活性物质产量相比，这种优化策略使活性物质产量提高了近2.8倍（4956.28 µg/ml）。抗真菌活性物质对黄曲霉具有较强的抗菌活性，最小抑菌浓度（MIC）和最小杀菌浓度（MFC）分别为3.13和6.25 μg/ml。关键词：吸水链霉菌BS-112，抑菌活性物质，Plackett-Burman设计，响应面法，中心组合设计。
Streptomyces hygroscopicus BS-112 was isolated from forest land on Mountain Tai, Shandong Province, China. This bacterium showed broad-spectrum antifungal and antibacterial activities. The medium that was necessary for the production of antifungal active substances from S. hygroscopicus BS-112 was optimized by the Plackett-Burman design (PBD) and response surface methodology (RSM). Initially, glucose, soybean powder and KH2PO4 were tested using the PBD and were found to be important factors that affected the production of active substances. The steepest ascent method was then used to determine the optimal region of each factor to include in the medium. The concentrations of important factors were subsequently optimized by using a central composite design (CCD). The results obtained demonstrated that the optimal media was composed of the following factors (g/l): corn meal, 5.00; glucose, 18.61; soybean powder, 31.15; KH2PO4, 0.38 and MgSO4, 0.85. This optimization strategy enhanced the production of active substances nearly 2.8 times (4956.28 µg/ml) compared to the yield of active substances that were produced using the basal medium. The antifungal active substances showed strong antimicrobial activity against Aspergillus flavus with minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of 3.13 and 6.25 µg/ml, respectively. Key word: Streptomyces hygroscopicus BS-112, antifungal active substances, Plackett-Burman design, response surface methodology, central composite design.