Proline is biosynthesized from arginine in Staphylococcus aureus.
Proline is biosynthesized from arginine in Staphylococcus aureus.
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DOI：
10.1099/13500872-142-6-1491
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发表时间：
1996-06
期刊：
影响因子：
1.5
通讯作者：
D. Townsend;A. Kaenjak;R. Jayaswal;B. Wilkinson
中科院分区：
文献类型：
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作者：
D. Townsend;A. Kaenjak;R. Jayaswal;B. Wilkinson
金黄色葡萄球菌（Staphylococcus aureus）NCTC 8325接种到缺乏脯氨酸的限定培养基中时表现出较长的滞后期（11 h），该滞后期可通过增加培养基中精氨酸的浓度或通过供应鸟氨酸来缩短。来自L-[14 C]精氨酸而非L-[14 C]谷氨酸的放射性掺入合并液代谢产物馏分中具有[14 C]脯氨酸色谱迁移率的斑点中。选择转座子Tn 917-lacZ突变体受损的精氨酸catalysts产生四个脯氨酸营养缺陷型。酶活性测定和前体补饲实验表明，S.金黄色葡萄球菌是通过鸟氨酸和δ ′-吡咯啉5-羧酸从精氨酸，而不是从谷氨酸。菌株8325 Pro+，一个脯氨酸原养型变体，通过在不存在脯氨酸的情况下培养8325获得，从培养基中积累L-[14 C]精氨酸的速率约为菌株8325的8倍，这表明其对脯氨酸饥饿的反应是增加精氨酸摄取。
Staphylococcus aureus NCTC 8325 exhibited a long lag phase (11 h) when inoculated into defined medium lacking proline, that could be shortened by increasing the concentration of arginine in the medium, or by supplying ornithine. Radioactivity from L-[14C]arginine, but not L-[14C]glutamate was incorporated into a spot with the chromatographic mobility of [14C]proline in the pool metabolites fraction. Selection for transposon Tn917-lacZ mutants impaired in arginine catabolism yielded four proline auxotrophs. Enzyme assays and precursor feeding experiments suggested that the major pathway for proline biosynthesis in S. aureus was from arginine via ornithine and delta'-pyrroline 5-carboxylate, rather than from glutamate. Strain 8325 Pro+, a proline prototrophic variant obtained by cultivation of 8325 in the absence of proline, accumulated L-[14C]arginine from the medium at about eight times the rate of strain 8325, suggesting its response to proline starvation was to increase arginine uptake.