Activation of calcium mobilization and calcium influx by alpha 1-adrenergic receptors in a smooth muscle cell line.
Activation of calcium mobilization and calcium influx by alpha 1-adrenergic receptors in a smooth muscle cell line.
复制标题
平滑肌细胞系中 α1-肾上腺素能受体激活钙动员和钙流入。
DOI：
10.1016/0006-291x(85)90463-2
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Dubyak,GR
中科院分区：
文献类型：
--
作者：
Reynolds,EE;Dubyak,GR
Alpha1-adrenergic receptor (α1R) mediated increases in the cytosolic levels of free Ca+2and the inositol phosphates were measured in a smooth muscle cell line, DDT1. Norepinephrine (NE) stimulated a rapid increase in cytosolic Ca+2by two distinct components: 1) release of Ca+2from intracellular sites (mobilization), and 2) influx of extracellular Ca+2. The mobilization component was not affected by removal of extracellular Ca+2or addition of La+3or Co+2to the buffer. The influx component was abolished by EGTA, La+3, or Co+2, but was not affected by the voltage-operated Ca+2channel blockers diltiazem or nifedipine. Depolarization of DDT1cells with 100 mM KCl or with gramicidin did not induce Ca+2influx. NE also increased inositol trisphosphate to 78% over basal levels within 1 minute. These results suggest that α1R on DDT1cells are coupled to both the mobilization of intracellular Ca+2and to receptor-operated Ca+2channels in the plasma membrane, and that polyphosphoinositide hydrolysis may play a role in these phenomena.
