Synthesis of Isoflavone Aglycones and Equol in Soy Milks Fermented by Food-Related Lactic Acid Bacteria and Their Effect on Human Intestinal Caco-2 Cells
Synthesis of Isoflavone Aglycones and Equol in Soy Milks Fermented by Food-Related Lactic Acid Bacteria and Their Effect on Human Intestinal Caco-2 Cells
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DOI：
10.1021/jf101513r
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发表时间：
2010-10-13
影响因子：
6.1
通讯作者：
Gobbetti, Marco
中科院分区：
文献类型：
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作者：
Di Cagno, Raffaella;Mazzacane, Francesco;Gobbetti, Marco
研究了从不同食物生态系统中分离的103株乳酸菌对对硝基苯- β - d -葡萄糖苷底物的（β -葡萄糖苷酶）活性。选择植物乳杆菌DPPMA24W和DPPMASL33、发酵乳杆菌DPPMA114和鼠李糖乳杆菌DPPMAAZ1作为混合发酵剂发酵各种豆浆制剂，其活性最高，主要是化学成分、蛋白质分散指数和大小尺寸不同。选用有机养殖大豆（OFS）制成的豆浆作为最佳制剂。所有选择的菌株在OFS豆浆中生长良好，达到几乎相同的细胞密度值（约8.5 log cfu/mL）。选用混合发酵剂发酵96 h后，OFS豆浆中M大豆素57.0 μ， M染料木素140.3 μ， M糖苷20.4 μ， M等量37.3 μ。采用发酵和非发酵OFS豆浆（或体外实验）对人肠道Caco-2/TC7细胞进行体外实验。发酵OFS豆浆可显著抑制干扰素- γ (ifn - γ) （1000 U/mL）和脂多糖（LPS） （100 ng/mL）诱导的Caco-2/TC7细胞炎症状态，即使受到干扰素- γ的负刺激也能保持紧密连接的完整性，并可显著抑制白细胞间素-1 β (2 ng/mL)处理后IL-8的合成。化学标准表明，这些影响是由于在发酵的OFS豆浆制剂中合成的异黄酮苷元，特别是雌马酚的伴随活性。
One hundred and three strains of lactic acid bacteria, isolated from various food ecosystems, were assayed for (beta-glucosidase activity toward p-nitrophenyl-beta-D-glucopyranoside substrate. Lactobacillus plantarum DPPMA24W and DPPMASL33, Lactobacillus fermentum DPPMA114, and Lactobacillus rhamnosus DPPMAAZ1 showed the highest activities and were selected as the mixed starter to ferment various soy milk preparations, which mainly differed for chemical composition, protein dispersibility index, and size dimension. The soy milk made with organically farmed soybeans (OFS) was selected as the best preparation. All selected strains grew well in OFS soy milk, reaching almost the same values of cell density (ca. 8.5 log cfu/mL). After 96 h of fermentation with the selected mixed starter, OFS soy milk contained 57.0 mu M daidzein, 140.3 mu M genistein, 20.4 mu M glycitein, and 37.3 mu M equal. Fermented and nonfermented OFS soy milks were used (or the in vitro assays on intestinal human Caco-2/TC7 cells. Fermented OFS soy milk markedly inhibited the inflammatory status of Caco-2/TC7 cells as induced by treatment with interferon-gamma (IFN-gamma) (1000 U/mL) and lipopolysaccharide (LPS) (100 ng/mL), maintained the integrity of the tight junctions, even if subjected to negative stimulation by IFN-gamma, and markedly inhibited the synthesis of IL-8, after treatment with interleukin-1 beta (2 ng/mL). As shown by using chemical standards, these effects were due to the concomitant activities of isoflavone aglycones and, especially, equol, which were synthesized in the fermented OFS soy milk preparation.