Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.
Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.
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海洋变暖和酸化对珊瑚礁珊瑚中卫城新成员共生建立和基因表达谱的影响
DOI：
10.3389/fmicb.2020.532447
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发表时间：
2020
影响因子：
5.2
通讯作者：
Huang H
中科院分区：
文献类型：
--
作者：
Sun Y;Jiang L;Gong S;Guo M;Yuan X;Zhou G;Lei X;Zhang Y;Yuan T;Lian J;Qian P;Huang H
共生关系的开始和大多数广播产卵珊瑚的早期发育在招募成功中起着关键作用，然而这些关键的早期阶段受到多种压力的威胁。然而，在海洋变暖和酸化的情况下，控制这些关键过程的分子机制仍然知之甚少。本研究研究了高温（~ 28.0°C和~ 30.5°C）和二氧化碳分压（~ 600和~ 1200 μatm）对中间Acropora稚虫33天早期发育和基因表达模式的交互影响。结果表明，高温、二氧化碳分压或综合处理对珊瑚存活率的影响为0.89%。值得注意的是，高温完全阻止了共生的成功建立和出芽过程，而酸化的影响可以忽略不计。共生关系的建立与出芽率呈正指数关系（y = 0.0004e6.43x, R = 0.72, P < 0.0001），说明共生关系在促进无性萌发中的重要作用。与控制温度（28°C）下的珊瑚相比，温度升高下的珊瑚虽然没有成功建立共生关系，但仍优先庇护Durusdinium spp.。此外，与对照相比，共生体和珊瑚宿主在实验条件下分别检测到351个和153个差异表达基因。在珊瑚宿主中，一些参与营养转运和组织荧光的基因受到高温的影响。在共生体中，与细胞生长、核糖体蛋白、光合作用和能量产生相关的一系列基因在高温下下调，这可能严重阻碍了内共生体细胞的成功增殖，并解释了共生关系建立失败的原因。因此，我们的研究结果表明，共生体对未来海洋条件的反应可能在塑造幼珊瑚成功的共生关系中起着至关重要的作用。
The onset of symbiosis and the early development of most broadcast spawning corals play pivotal roles in recruitment success, yet these critical early stages are threatened by multiple stressors. However, molecular mechanisms governing these critical processes under ocean warming and acidification are still poorly understood. The present study investigated the interactive impact of elevated temperature (∼28.0°C and ∼30.5°C) and partial pressure of carbon dioxide (pCO2) (∼600 and ∼1,200 μatm) on early development and the gene expression patterns in juvenile Acropora intermedia over 33 days. The results showed that coral survival was >89% and was unaffected by high temperature, pCO2, or the combined treatment. Notably, high temperature completely arrested successful symbiosis establishment and the budding process, whereas acidification had a negligible effect. Moreover, there was a positive exponential relationship between symbiosis establishment and budding rates (y = 0.0004e6.43x, R = 0.72, P < 0.0001), which indicated the importance of symbiosis in fueling asexual budding. Compared with corals at the control temperature (28°C), those under elevated temperature preferentially harbored Durusdinium spp., despite unsuccessful symbiosis establishment. In addition, compared to the control, 351 and 153 differentially expressed genes were detected in the symbiont and coral host in response to experimental conditions, respectively. In coral host, some genes involved in nutrient transportation and tissue fluorescence were affected by high temperature. In the symbionts, a suite of genes related to cell growth, ribosomal proteins, photosynthesis, and energy production was downregulated under high temperatures, which may have severely hampered successful cell proliferation of the endosymbionts and explains the failure of symbiosis establishment. Therefore, our results suggest that the responses of symbionts to future ocean conditions could play a vital role in shaping successful symbiosis in juvenile coral.
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影响因子：
3.5
作者：
Heyward, AJ;Negri, AP
通讯作者：
Negri, AP
影响因子：
3.7
作者：
Davies, Sarah W.;Ries, Justin B.;Castillo, Karl D.
通讯作者：
Castillo, Karl D.
影响因子：
3.6
作者：
Cerec, Virginie;Piquet-Pellorce, Claire;Bauche, Francoise
通讯作者：
Bauche, Francoise
影响因子：
2.7
作者：
Graham, Erin M.;Baird, Andrew H.;Connolly, Sean R.
通讯作者：
Connolly, Sean R.
DOI：
10.1098/rspb.2008.0180
发表时间：
2008-10-07
影响因子：
4.7
作者：
Abrego, David;Ulstrup, Karin E.;van Oppen, Madeleine J. H.
通讯作者：
van Oppen, Madeleine J. H.