Induction of antioxidant enzyme activity by hyperoxia (60 % O2) in the developing chick embryo
Induction of antioxidant enzyme activity by hyperoxia (60 % O2) in the developing chick embryo
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DOI：
10.1111/j.1469-7793.1998.289bo.x
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发表时间：
1998-05
期刊：
影响因子：
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通讯作者：
J. Golde;P. Borm;M. Wolfs;E. Rhijnsburger;C. Blanco
中科院分区：
文献类型：
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作者：
J. Golde;P. Borm;M. Wolfs;E. Rhijnsburger;C. Blanco
1早产时，与子宫内条件相比，人类和动物暴露在相对较高的氧气水平下，此时他们的抗氧化酶（AOE）系统仍然不成熟。以鸡胚为研究模型，研究了孵化期不同时间点高氧对AOE系统的影响。本文研究了鸡胚发育过程中（孵化期后半期）脑、心、肝、肠和肺等器官的AOE活性与高氧的关系。2将白色来航鸡种蛋分为对照组（n= 100）、试验组1（n= 80）、试验组2（n= 60）和试验组3（n= 30），分别暴露于60%O2中48 h，然后在第10、14和18天进行处理。超氧化物歧化酶（SOD），过氧化氢酶和谷胱甘肽过氧化物酶（GPx）的酶活性测定的脑，心，肝，肠和肺的匀浆。3在孵育期间的不同时间点暴露于高氧导致除大脑外的所有器官中SOD活性增加2至10倍。过氧化氢酶和GPx酶活性仅在实验组1中诱导，48 h后开始高氧。4高氧对发育中的鸡胚产生短暂的AOE诱导作用，其作用依赖于AOE、器官、孵育时间和暴露时间点。
1 At premature birth, man and animals are exposed to relatively high oxygen levels, compared with intra‐uterine conditions, at a time when their antioxidant enzyme (AOE) system is still immature. Using the chick embryo as a study model, we investigated changes in the AOE system in response to hyperoxia applied at different time points during the incubation period. Relations between hyperoxia and AOE activity were studied in selected organs (brain, heart, liver, intestine and lungs) of developing chick embryos (during the second half of the incubation period). 2 Incubated White Leghorn eggs were divided into four groups: control (n= 100) and three test groups exposed for 48 h to 60 % O2 on day 10 (test group 1, n= 80), day 14 (test group 2, n= 60) and day 18 (test group 3, n= 30). Superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) enzyme activities were measured in homogenates of the brain, heart, liver, intestine and lungs. 3 Exposure to hyperoxia at different time points during incubation resulted in a 2‐ to 10‐fold increase in SOD activity in all organs except the brain. Catalase and GPx enzyme activities were only induced in test group 1, 48 h after initiation of hyperoxia. 4 In the developing chick embryo, hyperoxia can produce a temporary induction of AOE activity, which is dependent on the AOE, organ, incubation time and time point of exposure.