Expression and clinical significance of CacyBP/SIP in pancreatic cancer
Expression and clinical significance of CacyBP/SIP in pancreatic cancer
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CacyBP/SIP在胰腺癌中的表达及临床意义
DOI：
10.1159/000151774
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发表时间：
2008-01-01
期刊：
影响因子：
3.6
通讯作者：
Fan, Daiming
中科院分区：
文献类型：
--
作者：
Chen, Xiong;Han, Guohong;Fan, Daiming
目的：钙周期蛋白结合蛋白或Siah-1相互作用蛋白(CacyBP/SIP)是泛素介导的蛋白分解过程中的一种成分，可与Skp1-CUL1-F box蛋白复合体结合，参与β-连环素的降解，与胃癌和肾癌的恶性表型有关。然而，CacyBP/SIP在胰腺癌进展中的作用仍不清楚。因此，本研究旨在探讨CacyBP/SIP在胰腺癌中的表达及其临床意义。方法：对胰腺癌石蜡包埋切片和正常胰腺组织进行免疫组织化学染色。采用Western印迹和半定量RT-PCR方法检测8对胰腺癌及其癌旁组织中CacyBP/SIPmRNA和蛋白的表达。结果：CacyBP/SIP在胰腺癌组织中的表达显著升高(28/68或41.2%)，并与分化程度、较高的TNM分期(肿瘤、淋巴结、转移)和远处转移有关。此外，与癌旁组织相比，几乎所有的癌组织中CacyBP/SIPmRNA和蛋白的表达水平都较高。结论：CacyBP/SIP蛋白可能在胰腺癌的发生过程中起重要作用，其高水平表达可能与胰腺癌的恶性潜能有关。版权所有(C)2008 S.Karger AG，巴塞尔和IAP。
Aims: Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of the ubiquitin-mediated proteolysis, could bind SKP1-CUL1-F box protein complex and participate in beta-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer and renal cancer. However, the role of CacyBP/SIP in pancreatic cancer progression still remains unclear. Therefore, the aim of the present study was to investigate the expression and clinical significance of CacyBP/SIP in pancreatic cancer. Methods: Immunohistochemistry was carried out on paraffin-embedded sections of pancreatic cancer and normal pancreatic tissues. In addition, Western blot and semiquantitative RT-PCR were carried out to analyze mRNA and protein expression of CacyBP/SIP in 8 pairs of freshly resected pancreatic cancer and their adjacent nontumorous tissue. Results: CacyBP/SIP expression was significantly increased in pancreatic cancer tissue (28/68 or 41.2%) and correlated with differentiation degree, higher TNM ( tumor, node, metastasis) stage and distance metastasis. Also, mRNA and protein expression of CacyBP/SIP were found to be at higher levels in almost all cancer tissues compared to adjacent tissues. Conclusions: CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer. Copyright (C) 2008 S. Karger AG, Basel and IAP.