Glc8, a regulator of protein phosphatase type 1, mediates oxidation tolerance, asexual development and virulence in Beauveria bassiana, a filamentous entomopathogenic fungus
Glc8, a regulator of protein phosphatase type 1, mediates oxidation tolerance, asexual development and virulence in Beauveria bassiana, a filamentous entomopathogenic fungus
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Glc8 是 1 型蛋白磷酸酶的调节剂，介导白僵菌（一种丝状昆虫病原真菌）的氧化耐受性、无性发育和毒力
DOI：
10.1007/s00294-018-0876-y
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发表时间：
2019-02-01
期刊：
影响因子：
2.5
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
Peng, Yue-Jin;Ding, Jin-Li;Ying, Sheng-Hua
蛋白磷酸酶1型（PP1）在酵母细胞代谢和发育中起重要作用。在PP1酶复合体中，Glc8蛋白是一个全局调控亚基，调控着许多生理过程。然而，其在丝状真菌中的生物学作用尚未被探索。在这项研究中，我们鉴定了球孢白僵菌（Beauveria bassiana）中Glc8的酵母同源物。BbGlc8基因的破坏对营养生长没有显著影响，但导致突变体的分生率（51%）和囊胚产量（55%）显著降低。通过角质层感染和血内注射试验表明，ΔBbGlc8突变体对氧化应激的敏感性增强，毒力减弱。转录组学分析表明，BbGlc8在分生过程中调控的基因主要与代谢、细胞拯救和细胞壁形成相关。值得注意的是，在ΔBbGlc8突变体中，BbOsmC2 （OsmC蛋白家族成员）作为一个下调基因参与了真菌对盐胁迫的抗性、孢子分化和毒力。因此，BbOsmC2在孢子分化过程中作为BbGlc8的下游靶点，而不是在应激反应中起作用。我们的研究结果表明，BbGlc8通过介导综合遗传途径对球孢白僵菌的生物防治潜力起作用。
Protein phosphatase type 1 (PP1) plays an important role in cellular metabolism and development in yeast. In PP1 enzyme complex, Glc8 protein is a global regulatory subunit and regulates many physiological processes. However, its biological roles are unexplored in filamentous fungi. In this study, we characterized a yeast ortholog of Glc8 in Beauveria bassiana, a filamentous entomopathogenic fungus. Gene disruption of BbGlc8 had no significant effect on vegetative growth, but resulted in a significant reduction in conidiation (51%) and blastospore yield (55%) in the mutant. The ΔBbGlc8 mutant displayed an enhanced sensitivity to oxidative stress and a weakened virulence as indicated by cuticle infection and intrahemocoel injection assays. Transcriptomic analysis indicated that the genes regulated by BbGlc8 during conidiation were primarily associated with metabolism, cell rescue and cell wall formation. Notably, as a down-regulated gene in ΔBbGlc8 mutant, BbOsmC2 (a member of OsmC protein family) contributes to fungal resistance to salt stress, spore differentiation and virulence. Thus, BbOsmC2 functions as a down-stream target of BbGlc8 during spore differentiation, but not in stress response. Our findings indicate that BbGlc8 contributes to the biocontrol potential of B. bassiana by mediating comprehensive genetic pathways.