Antigenic classification of Rickettsia felis by using monoclonal and polyclonal antibodies
Antigenic classification of Rickettsia felis by using monoclonal and polyclonal antibodies
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DOI：
10.1128/cdli.10.2.221-228.2003
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发表时间：
2003-03-01
期刊：
影响因子：
--
通讯作者：
Raoult, D
中科院分区：
文献类型：
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作者：
Fang, R;Raoult, D
猫立克次体是一种由跳蚤传播的立克次体。在其系统发育鉴定和表型鉴定之间存在差异。在首次报告猫雷氏体后，血清学试剂检测认为它与另一种公认的跳蚤传播的立克次体伤寒雷氏体密切相关。随后，通过遗传分析，它似乎与斑点热组（SFG）立克次体有更密切的关系。本研究采用微免疫荧光（MIF）血清学分型和单克隆抗体（mab）对猫鼠进行了研究。小鼠恙螨多克隆抗血清仅与SFG立克次体交叉反应。基于MIF的邻域连接分析表明，狐耳鼠与SFG立克次体存在亲缘关系，与澳洲鼠、阿卡瑞鼠和山地鼠具有聚类关系。针对120 kda蛋白抗原或17 kda多肽，构建了21个单抗。它们与SFG立克次体的大多数成员发生交叉反应，但与斑疹伤寒组（TG）立克次体的proproazekir， typhi r或加拿大r没有交叉反应。先前生成的64种针对其他7种立克次体的单克隆抗体在狐猴身上进行了测试。3个单克隆抗体与120 kda抗原反应，分别由非洲r.a africae、conorii r.a akari产生。它们表现出与狸鼠的交叉反应。我们所有的数据都表明，狐猴具有SFG立克次体的抗原谱。
Rickettsia felis is a flea-transmitted rickettsia. There is a discrepancy between its reported phylogenic and phenotypic identifications. Following the first report of R. felis, it was considered by tests with serologic reagents to be closely related to another recognized flea-transmitted rickettia, R. typhi. Subsequently, it appeared to be more closely related to spotted fever group (SFG) rickettsiae by genetic analysis. In the present work, R.felis was studied by microimmunofluorescence (MIF) serologic typing and with monoclonal antibodies (MAbs). Mouse polyclonal antisera to R. felis cross-reacted only with SFG rickettsiae. A neighbor-joining analysis based on MIF indicated that R.felis is actually related to SFG rickettsiae antigenically, clustering with R. australis, R. akari, and R. montanensis. A panel of 21 MAbs was raised against a 120-kDa protein antigen or a 17-kDa polypeptide of R. felis. They cross-reacted with most members of the SFG rickettsiae but not with R. prowazekii, R. typhi, or R. canadensis of the typhus group (TG) rickettsiae. Sixty-four MAbs previously generated to seven other ricketttsial species were tested with R. felis. Three MAbs reacted with the 120-kDa antigen and were generated by R. africae, R. conorii, and R. akari, respectively. They exhibited cross-reactivities with R. felis. All our data show that R. felis harbors the antigenic profile of an SFG rickettsia.