Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children.
Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children.
复制标题
卡氏肺囊虫感染：正常儿童和免疫抑制儿童中患病率较高的证据。
作者：
L. Pifer;W. Hughes;S. Stagno;D. Woods
用鸡胚肺上皮细胞培养三代卡氏肺孢子虫，制备了卡氏肺孢子虫特异性抗原和抗血清，用于免疫电泳和间接免疫荧光抗体技术。结果表明，该方法对卡氏肺孢子虫肺炎（PCP）患者血清中卡氏肺孢子虫抗原和抗体的检测具有较高的特异性和敏感性。在95%的PCP患者中检测到抗原血症，在15%的无肺炎的癌症患者中检测到抗原血症，在正常儿童中检测不到抗原血症。在对正常婴儿和儿童的横断面和纵向研究中，卡氏肺孢子虫血清抗体的获得被证明随着年龄的增加而进行性发生。到4岁时，三分之二的正常儿童被发现具有卡氏肺孢子虫抗体，滴度为1：16或更高。这些研究表明，亚临床卡氏肺孢子虫感染在正常儿童中非常普遍，类似于其他机会性感染，其中活动性疾病主要表现在受损宿主中。
Using Pneumocystis carinii organisms propagated through three passages in embryonic chick epithelial lung cultures, specific antigens and antisera were prepared for use in counterimmunoelectrophoresis and indirect immunofluorescent antibody techniques. These methods proved to be specific and sensitive for the detection of P. carinii antigen and antibody, respectively, in sera, and were applied to the study of cancer patients with P. carinii pneumonitis (PCP), cancer patients without pneumonitis, and normal children. Antigenemia was detected in 95% of patients with PCP, in 15% of cancer patients without pneumonitis, and in none of the normal children tested. In cross-sectional and longitudinal studies of normal infants and children, acquisition of serum antibody to P. carinii was demonstrated to occur progressively with increase in age. By 4 years of age two thirds of the normal children were found to have antibody to P. carinii in titers of 1:16 or greater. These studies indicate that subclinical P. carinii infection is highly prevalent in normal children, analogous to other opportunistic infections where active disease is manifest predominantly in the compromised host.