Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells
Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells
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DOI：
10.1177/1535370213497173
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发表时间：
2013-08-01
影响因子：
3.2
通讯作者：
Kim, Chan-Wha
中科院分区：
文献类型：
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作者：
Kim, Hyun-Jung;Jung, Jessica;Kim, Chan-Wha
极低频电磁场（ELF-EMF）影响许多生物功能，如基因表达，细胞命运决定甚至细胞分化。为了研究ELF-EMF暴露与分化之间的相关性，骨髓间充质干细胞（BM-MSCs）在体外扩增期间经受50-Hz电磁场。通过一系列不同的分析方法分析ELF-EMF对BM-MSC的影响，以了解其在增强神经分化中的作用。ELF-EMF暴露显着降低增殖率，这反过来又导致神经元分化的增加。ELF-EMF处理的细胞显示神经元分化标记物（MAP 2）的水平增加，而早期神经元标记物（Nestin）的水平下调。此外，在二维电泳图谱中检测到8个差异表达蛋白，并使用ESI-Q-TOF LC/MS/MS进行鉴定。其中，铁蛋白轻链，硫氧还蛋白依赖性过氧化物还原酶，微管蛋白β-6链在ELF-EMF刺激组中上调。铁蛋白和硫氧还蛋白依赖性过氧化物还原酶参与多种功能，包括Ca 2+调节，这是神经变性的关键组成部分。我们还观察到，极低频电磁场暴露后细胞内Ca 2+含量显着升高，这增强了铁蛋白和硫氧还蛋白依赖性过氧化物还原酶在分化过程中的调节作用。值得注意的是，蛋白质印迹分析表明ELF-EMF刺激组中铁蛋白轻链的表达显著增加（0.60 vs. 1.08; P < 0.01）。这些蛋白质可能有助于了解ELF-EMF刺激对神经分化过程中BM-MSCs的影响及其作为治疗神经退行性疾病的临床治疗选择的潜在用途。
Extremely low-frequency electromagnetic fields (ELF-EMF) affect numerous biological functions such as gene expression, cell fate determination and even cell differentiation. To investigate the correlation between ELF-EMF exposure and differentiation, bone marrow derived mesenchymal stem cells (BM-MSCs) were subjected to a 50-Hz electromagnetic field during in vitro expansion. The influence of ELF-EMF on BM-MSCs was analysed by a range of different analytical methods to understand its role in the enhancement of neural differentiation. ELF-EMF exposure significantly decreased the rate of proliferation, which in turn caused an increase in neuronal differentiation. The ELF-EMF-treated cells showed increased levels of neuronal differentiation marker (MAP2), while early neuronal marker (Nestin) was down-regulated. In addition, eight differentially expressed proteins were detected in two-dimensional electrophoresis maps, and were identified using ESI-Q-TOF LC/MS/MS. Among them, ferritin light chain, thioredoxin-dependent peroxide reductase, and tubulin beta-6 chain were up-regulated in the ELF-EMF-stimulated group. Ferritin and thioredoxin-dependent peroxide reductase are involved in a wide variety of functions, including Ca2+ regulation, which is a critical component of neurodegeneration. We also observed that the intracellular Ca2+ content was significantly elevated after ELF-EMF exposure, which strengthens the modulatory role of ferritin and thioredoxin-dependent peroxide reductase, during differentiation. Notably, western blot analysis indicated significantly increased expression of the ferritin light chain in the ELF-EMF-stimulated group (0.60 vs. 1.08; P < 0.01). These proteins may help understand the effect of ELF-EMF stimulation on BM-MSCs during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.