Whole genome sequencing and biological characterization of Duck/JS/10, a new lentogenic class I Newcastle disease virus
Whole genome sequencing and biological characterization of Duck/JS/10, a new lentogenic class I Newcastle disease virus
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DOI：
10.1007/s00705-012-1248-4
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发表时间：
2012-05-01
影响因子：
2.7
通讯作者：
Ding, Chan
中科院分区：
文献类型：
--
作者：
Meng, Chunchun;Qiu, Xvsheng;Ding, Chan
从我国1只未免疫的鸭体内分离到1株弱原性纽卡斯尔病病毒Duck/JS/10（JS 10）。该病毒的完整基因组包含15，198个核苷酸。根据基因组的长度和F基因的部分序列，该病毒被分类为I类基因型4 NDV。通过血凝抑制（HI）、病毒中和（VN）试验和动物实验，比较了该病毒与新城疫病毒La Sota株的抗原性。我们的结果表明，JS 10产生更高的HI和VN滴度比La Sota对I类和II类强毒株。动物实验表明，与接种La Sota疫苗的鸡相比，接种JS 10疫苗的鸡的病毒脱落显著减少。总体而言，这项研究强烈表明，JS 10可能有资格作为一个新的候选疫苗对纽卡斯尔病。
A lentogenic Newcastle disease virus (NDV), Duck/JS/10 (JS10), was isolated from an unvaccinated duck in China. The complete genome of the virus contained 15,198 nucleotides. Based on length of the genome and a partial sequence of the F gene, the virus was classified as a class I genotype 4 NDV. The antigenicity of the virus was compared with that of NDV strain La Sota via hemagglutination inhibition (HI), virus neutralization (VN) assay and animal experiments. Our results show that JS10 generates higher HI and VN titers than La Sota against both class I and II virulent NDV strains. Experiments on animals demonstrate that virus shedding from chickens vaccinated with JS10 is significantly reduced when compared to those vaccinated with La Sota. Overall, this study strongly suggests that JS10 may qualify as a new vaccine candidate against Newcastle disease.