Mapping of Membrane Lipid Order in Root Apex Zones of Arabidopsis thaliana.
Mapping of Membrane Lipid Order in Root Apex Zones of Arabidopsis thaliana.
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拟南芥根尖区膜脂顺序图谱
DOI：
10.3389/fpls.2015.01151
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发表时间：
2015
影响因子：
5.6
通讯作者：
Wan Y
中科院分区：
文献类型：
--
作者：
Zhao X;Zhang X;Qu Y;Li R;Baluška F;Wan Y
在这项研究中，我们使用的荧光探针，Di-4-ANEPPDHQ，映射的膜脂顺序在拟南芥根的顶端区域的分布。Di-4-ANEPPDHQ染色根的广义极化（GP）值表明根过渡区（RTZ）的脂质顺序最高。皮层细胞的脂质有序度高于同一区域的表皮细胞，而发育中的根毛细胞极性非常明显，顶端区域的脂质有序度较高。此外，内体具有较低的脂质顺序比质膜（PM）。布雷菲德菌素A（BFA）处理降低了RTZ中表皮细胞质膜和内体膜中的脂质顺序。在表皮细胞中，经BFA处理后，BFA诱导的隔室的脂质顺序变得高于PM。而极性生长的根毛顶端则没有表现出同样的行为。PM的脂质顺序保持不变，具有比内体更高的值。这表明，在PM中的脂质排序的影响内体囊泡的再循环在表皮细胞的根尖过渡区，但不是在根毛的拟南芥。
In this study, we used the fluorescence probe, Di-4-ANEPPDHQ, to map the distribution of membrane lipid order in the apical region of Arabidopsis roots. The generalized polarization (GP) value of Di-4-ANEPPDHQ-stained roots indicated the highest lipid order in the root transition zone (RTZ). The cortical cells have higher lipid order than the epidermal cells in same regions, while the developing root hairs show very prominent cell polarity with high lipid order in apical region. Moreover, the endosomes had lower lipid order than that of the plasma membrane (PM). Brefeldin A (BFA) treatment decreased the lipid order in both the plasma and endosomal membranes of epidermal cells in the RTZ. The lipid order of BFA-induced compartments became higher than that of the PM after BFA treatment in epidermal cells. Meanwhile, the polarly growing tips of root hairs did not show the same behavior. The lipid order of the PM remained unchanged, with higher values than that of the endosomes. This suggests that the lipid ordering in the PM was affected by recycling of endosomal vesicles in epidermal cells of the root apex transition zone but not in the root hairs of Arabidopsis.