Asymmetric Rab11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system
Asymmetric Rab11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system
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DOI：
10.1016/j.cell.2005.08.017
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发表时间：
2005-09-09
期刊：
影响因子：
64.5
通讯作者：
Knoblich, JA
中科院分区：
文献类型：
--
作者：
Emery, G;Hutterer, A;Knoblich, JA
果蝇感觉器官前体（SOP）细胞是一个良好的非对称细胞分裂模型系统。在SOP分裂期间，决定因素麻木和神经化的分离为PIIB子细胞，并通过调节Notch/Delta信号传导来建立独特的细胞命运。在这里，我们描述了一种麻木和神经化的非依赖性机制，该机制在细胞命令规范中有用。我们表明，在两个子细胞中，槽口配体三角洲的贩运是不同的。在PIIB，三角洲通过以Rab11为标志的回收内体。但是，在PIIA中，回收内体不会形成，因为中心体未能募集核辐射，核辐射是Rab11结合伴侣，对于回收内体形成至关重要。使用哺乳动物细胞培养系统，我们证明了回收内体对于三角洲活性至关重要。我们的结果表明，细胞可以通过抑制单个内吞室的形成来调节信号通路并影响其发育命运。
Drosophila sensory organ precursor (SOP) cells are a well-studied model system for asymmetric cell division. During SOP division, the determinants Numb and Neuralized segregate into the pIIb daughter cell and establish a distinct cell fate by regulating Notch/Delta signaling. Here, we describe a Numb- and Neuralized-independent mechanism that acts redundantly in cell-fate specification. We show that trafficking of the Notch ligand Delta is different in the two daughter cells. In pIIb, Delta passes through the recycling endosome which is marked by Rab11. In pIIa, however, the recycling endosome does not form because the centrosome fails to recruit Nuclear fallout, a Rab11 binding partner that is essential for recycling endosome formation. Using a mammalian cell culture system, we demonstrate that recycling endosomes are essential for Delta activity. Our results suggest that cells can regulate signaling pathways and influence their developmental fate by inhibiting the formation of individual endocytic compartments.