Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells
Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells
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DOI：
10.3109/13813455.2013.768270
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发表时间：
2013-05-01
影响因子：
3
通讯作者：
Eckel, Juergen
中科院分区：
文献类型：
--
作者：
Goergens, Sven W.;Raschke, Silja;Eckel, Juergen
卵泡抑素样蛋白 1 (Fstl1) 是卵泡抑素家族的分泌型糖蛋白。 Fstl1 由 C2C12 细胞分泌，骨骼肌中 Akt1 的过度表达会导致其在肌肉中的诱导并增加循环水平。迄今为止，尚未研究人类肌管分泌 Fstl1 以及运动对其循环水平的影响。在这里，我们检查了原代人骨骼肌细胞中 Fstl1 表达和分泌的调节以及急性运动对人类 Fstl1 血清浓度的影响。我们发现人类肌管以分化依赖性方式表达和分泌 Fstl1。此外，IFN γ 和 IL-1 β 显着增加 Fstl1 分泌。电脉冲刺激 (EPS) 诱导的肌管收缩活动不调节 Fstl1。有趣的是，我们观察到 60 分钟的循环使血清 Fstl1 水平增加了 22%。总之，我们证明 Fstl1 由人类肌管表达和分泌，并且运动后血浆 Fstl1 水平升高。
Follistatin-like protein 1 (Fstl1) is a secreted glycoprotein of the follistatin family. Fstl1 is secreted by C2C12 cells, and Akt1 over-expression in skeletal muscle leads to its induction in muscle and increased circulating levels. So far, secretion of Fstl1 by human myotubes and the effect of exercise on its circulating levels have not been investigated. Here, we examined both the regulation of Fstl1 expression and secretion in primary human skeletal muscle cells and the effect of acute exercise on Fstl1 serum concentrations in humans. We show that human myotubes express and secrete Fstl1 in a differentiation-dependent manner. Furthermore, IFN gamma and IL-1 beta significantly increase Fstl1 secretion. Electrical pulse stimulation (EPS)-induced contractile activity of myotubes did not regulate Fstl1. Interestingly, we observed that 60 min cycling increased serum Fstl1 level by 22%. In conclusion, we demonstrate that Fstl1 is expressed and secreted by human myotubes and plasma Fstl1 levels are increased after exercise.