Advantage of Florisil over charcoal separation in a mechanized testosterone radioimmunoassay.
Advantage of Florisil over charcoal separation in a mechanized testosterone radioimmunoassay.
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在机械化睾酮放射免疫测定中，弗洛里硅土相对于木炭分离的优势。
DOI：
10.1093/clinchem/26.11.1613
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发表时间：
1980
影响因子：
9.3
通讯作者：
A. Kowarski
中科院分区：
文献类型：
--
作者：
S. Harman;P. Tsitouras;M. A. Kowatch;A. Kowarski
我们通过机械化每个移液步骤，并通过在多管涡旋混合器中用活性硅酸镁（Florisil）摇动测定管，分离结合激素和游离激素，促进了睾酮的放射免疫测定。与右旋糖酐-木炭分离相比，Florisil分离的优点是，除了在主动摇动期间，睾酮仅被可忽略不计地吸附，因此添加吸附剂的间隔不会在一系列的第一个和最后一个试管之间产生结合激素估计值的差异。对于每升含有4.0杯睾酮的样品，我们的方法给出了5%（测定内）和6%（测定间）的CVs，明显优于用木炭获得的CVs（分别为12%和15%）。该方法空白值低，准确度高。
We have facilitated radiommunoassay of testosterone by mechanizing every pipetting step and separating bound from free hormone by shaking assay tubes with activated magnesium silicate (Florisil) in a multi-tube Vortex mixer. Compared with dextran-charcoal separation, Florisil separation has the advantage that testosterone is only negligibly adsorbed except during active shaking, so that the interval for adding adsorbent does not produce discrepancies in bound hormone estimates between first and last assay tubes in a series. Our method gave CVs of 5% (within assay) and 6% (between assay) for samples containing 4.0 mug of testosterone per liter, considerably better than those obtained with charcoal (respectively 12 and 15%). The assay also had low blank values and demonstrated excellent accuracy.