A new L-arabinose isomerase with copper ion tolerance is suitable for creating protein-inorganic hybrid nanoflowers with enhanced enzyme activity and stability
A new L-arabinose isomerase with copper ion tolerance is suitable for creating protein-inorganic hybrid nanoflowers with enhanced enzyme activity and stability
复制标题
一种具有铜离子耐受性的新型L-阿拉伯糖异构酶适用于创建具有增强的酶活性和稳定性的蛋白质-无机杂化纳米花
作者：
Zheng Xu;Rui Wang;Chao Liu;Bo Chi;Jian Gao;Beining Chen;Hong Xu
使用Cu 2+、PBS缓冲液和源自多粘类芽孢杆菌（Paenibacillus polymyxa，PPAI）的铜离子耐受性L-阿拉伯糖异构酶制备蛋白质-无机杂化纳米花。利用这些纳米花分别以L-阿拉伯糖或D-半乳糖为底物制备了昂贵的稀有糖L-核酮糖或D-塔格糖。PPAI杂交纳米花在热和酸性条件下均表现出更好的酶稳定性。同时，使用这些混合纳米花的长期批量生产是成功的，显示出高稳定性，其实现了从L-阿拉伯糖到L-核酮糖的61.8%的转化率。
Protein–inorganic hybrid nanoflowers were prepared using Cu2+, PBS buffer, and a copper ion tolerant L-arabinose isomerase that was derived from Paenibacillus polymyxa (PPAI). The expensive rare sugar L-ribulose or D-tagatose was prepared utilizing these nanoflowers with L-arabinose or D-galactose as the substrate, respectively. The PPAI hybrid nanoflowers showed better enzyme stabilities in both thermal and acidic conditions. Meanwhile, long term batch production was successful showing high stability using these hybrid nanoflowers, which achieved a conversion rate of 61.8% from L-arabinose to L-ribulose.