Emerging technologies for non-invasive quantification of physiological oxygen transport in plants
Emerging technologies for non-invasive quantification of physiological oxygen transport in plants
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DOI：
10.1007/s00425-013-1926-9
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发表时间：
2013-09-01
期刊：
影响因子：
4.3
通讯作者：
McLamore, E. S.
中科院分区：
文献类型：
--
作者：
Chaturvedi, P.;Taguchi, M.;McLamore, E. S.
氧在植物代谢、胁迫响应/信号传导和适应环境变化中起关键作用（Lambers和Colmer，Plant Soil 274：7-15，2005; Pitzschke等人，Antioxid Redox Signal 8：1757-1764，2006;货车Breusegem等人，Plant Sci 161：405-414，2001）。活性氧（ROS）是机体对环境胁迫的适应性反应过程中产生的一种代谢副产物。虽然对植物对胁迫的适应了解很多（例如，解毒酶，抗氧化剂的产生），ROS代谢，O-2运输和应激反应机制之间的联系是未知的。因此，测量O-2的非侵入性技术对于理解生理O-2转运和ROS信号传导之间的联系至关重要。新的非侵入性技术允许在单细胞甚至细胞器水平上实时测量O-2。本综述简要总结了目前可用的（即，主流）技术测量O-2，然后介绍新兴技术测量O-2。先进的技术提供了非侵入性的能力（即，非破坏性地）测量O-2被突出显示。在不久的将来，这些非侵入式传感器将促进新的实验，使植物生理学家能够提出新的假设驱动的研究问题，旨在提高我们对生理O-2运输的理解。
Oxygen plays a critical role in plant metabolism, stress response/signaling, and adaptation to environmental changes (Lambers and Colmer, Plant Soil 274:7-15, 2005; Pitzschke et al., Antioxid Redox Signal 8:1757-1764, 2006; Van Breusegem et al., Plant Sci 161:405-414, 2001). Reactive oxygen species (ROS), by-products of various metabolic pathways in which oxygen is a key molecule, are produced during adaptation responses to environmental stress. While much is known about plant adaptation to stress (e.g., detoxifying enzymes, antioxidant production), the link between ROS metabolism, O-2 transport, and stress response mechanisms is unknown. Thus, non-invasive technologies for measuring O-2 are critical for understanding the link between physiological O-2 transport and ROS signaling. New non-invasive technologies allow real-time measurement of O-2 at the single cell and even organelle levels. This review briefly summarizes currently available (i.e., mainstream) technologies for measuring O-2 and then introduces emerging technologies for measuring O-2. Advanced techniques that provide the ability to non-invasively (i.e., non-destructively) measure O-2 are highlighted. In the near future, these non-invasive sensors will facilitate novel experimentation that will allow plant physiologists to ask new hypothesis-driven research questions aimed at improving our understanding of physiological O-2 transport.