Truncated human endothelin receptor A produced by alternative splicing and its expression in melanoma.
Truncated human endothelin receptor A produced by alternative splicing and its expression in melanoma.
复制标题
截短的人内皮蛋白受体A由替代剪接产生的A及其在黑色素瘤中的表达产生。
DOI：
10.1038/bjc.1998.643
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发表时间：
1998-11
影响因子：
8.8
通讯作者：
Carter, N D
中科院分区：
文献类型：
--
作者：
Zhang, Y F;Jeffery, S;Burchill, S A;Berry, P A;Kaski, J C;Carter, N D
本研究采用逆转录酶聚合酶链反应扩增人内皮素受体 A (ETA) 和 ETB 受体 mRNA。发现了外显子 3 和 4 被跳过的截短 ETA 受体转录本。这两个外显子的跳过导致 109 个氨基酸从受体中删除。截短的受体在所有检查的组织和细胞中都有表达，但表达水平各不相同。在黑色素瘤细胞系和黑色素瘤组织中，截短型受体基因是主要种类，而野生型ETA在其他组织中占主导地位。通过 Northern blot 在黑色素瘤细胞系中鉴定出 1.9 kb ETA 转录本，该转录本比之前报道的心脏和其他组织中的转录本 (4.3 kb) 小得多。截短型和野生型ETA受体的cDNA编码区被稳定转染到中国仓鼠卵巢(CHO)细胞中。截短的ETA受体转染的CHO细胞不显示与内皮素1 (ET-1)或内皮素3 (ET-3)的结合亲和力。这种截短的 ETA 受体的功能和生物学意义尚不清楚，但它可能对细胞对 ET 的反应具有调节作用。
In this study, reverse transcriptase polymerase chain reaction was used to amplify human endothelin receptor A (ETA) and ETB receptor mRNA. A truncated ETA receptor transcript with exons 3 and 4 skipped was found. The skipping of these two exons results in 109 amino acids being deleted from the receptor. The truncated receptor was expressed in all tissues and cells examined, but the level of expression varied. In melanoma cell lines and melanoma tissues, the truncated receptor gene was the major species, whereas the wild-type ETA was predominant in other tissues. A 1.9-kb ETA transcript was identified in melanoma cell lines by Northern blot, which was much smaller than the transcript in heart and in other tissues reported previously (4.3 kb). The cDNA coding regions of the truncated and wild-type ETA receptors were stably transfected into Chinese hamster ovary (CHO) cells. The truncated ETA receptor-transfected CHO cells did not show binding affinity to endothelin 1 (ET-1) or endothelin 3 (ET-3). The function and biological significance of this truncated ETA receptor is not clear, but it may have regulatory roles for cell responses to ETs.