Alternate radiolabeled markers for detecting metabolic activity of Mycobacterium leprae residing in murine macrophages
Alternate radiolabeled markers for detecting metabolic activity of Mycobacterium leprae residing in murine macrophages
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用于检测鼠巨噬细胞中麻风分枝杆菌代谢活性的替代放射性标记标记
DOI：
10.1128/jcm.21.5.861-864.1985
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发表时间：
1985
影响因子：
9.4
通讯作者：
R. Hastings
中科院分区：
文献类型：
--
作者：
H. K. Prasad;R. Hastings
这项研究证明了使用 4% NaOH 作为小鼠巨噬细胞溶解剂来区分宿主巨噬细胞代谢和细胞内麻风分枝杆菌代谢的效用。 4% 浓度的 NaOH 对标记的分枝杆菌没有有害影响。因此，可以对细胞内麻风分枝杆菌代谢活性的替代放射性标记指标进行实验。与含有高压灭菌杆菌的对照培养物相比，在含有新鲜提取的（“活的”）麻风分枝杆菌菌株的培养物中观察到14C-氨基酸混合物、[14C]亮氨酸、[14C]尿苷和无载体32P的显着掺入。
This study demonstrated the utility of using 4% NaOH as a murine macrophage cell-solubilizing agent to discriminate between host macrophage metabolism and that of intracellular Mycobacterium leprae. A 4% concentration of NaOH had no deleterious effect on labeled mycobacteria. Thereby, alternate radiolabeled indicators of the metabolic activity of intracellular M. leprae could be experimented with. Significant incorporation of 14C-amino acid mixture, [14C]leucine, [14C]uridine, and carrier-free 32P was observed in cultures containing freshly extracted ("live") strains of M. leprae as compared with control cultures containing autoclaved bacilli.