REACTION OF PROTEINS WITH GLUTARALDEHYDE
REACTION OF PROTEINS WITH GLUTARALDEHYDE
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DOI：
10.1016/0003-9861(68)90554-7
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发表时间：
1968-01-01
影响因子：
3.9
通讯作者：
HIRAMOTO, R
中科院分区：
文献类型：
--
作者：
HABEEB, AFS;HIRAMOTO, R
戊二醛可以与氨基酸的o-氨基、某些肽的n端氨基和半胱氨酸的巯基发生反应。酪氨酸和组氨酸衍生物的酚环和咪唑环部分反应。戊二醛与蛋白质如牛血清白蛋白、卵白蛋白和人γ球蛋白主要与赖氨酸的[ε]-氨基反应，形成主要的分子间交联。然而，酪氨酸、组氨酸和巯基残基之间确实发生了一些反应。可溶性聚集蛋白能够与针对其各自天然蛋白的抗体发生反应。戊二醛还能偶联卵清蛋白和牛血清白蛋白，产品中只含有少量的卵清蛋白和牛血清白蛋白的聚集污染物。
Glutaraldehyde was found to react with the o-amino groups of amino acids, the N-terminal amino groups of some peptides and the sulfhydryl group of cysteine. The phenolic and the imidazole rings of tyrosine and histidine derivatives were partially reactive. With proteins such as bovine serum albumin, ovalbumin and human gamma globulin, glutaraldehyde reacted predominantly with the [epsilon]-amino groups of lysine to form mainly intermolecular cross-linkages. Some reaction, however, did occur with tyrosine, histidine, and sulfhydryl residues. The soluble aggregated proteins were capable of reacting with antibodies against their respective native proteins. Glutaraldehyde was also capable of conjugating ovalbumin to bovine serum albumin and the product was found to contain only minor contaminants of aggregated ovalbumin and bovine serum albumin.