Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida).
Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida).
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Tintinnid 纤毛虫（纤毛纲、螺毛纲、脉毛纲）的三个基于 rDNA 位点的系统发育。
DOI：
10.1111/jeu.12354
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Gong J
中科院分区：
文献类型：
--
作者：
Zhang Q;Agatha S;Zhang W;Dong J;Yu Y;Jiao N;Gong J
为了更好地了解丁丁纲纤毛虫的多样性、系统发育和进化，有必要将多个分子标记与正确鉴定和记录的形态种联系起来。据此，收集并分析了主要来自黄海和东海的54种丁丁属形态种/分离种。采用单细胞法，获得了3个rDNA位点（18S, ITS1-5.8S-ITS2, 28S D1-D5区）的序列。通过显微照片、测量、形态学描述，并与原始物种描述进行了比较，记录了26个丁丁属形态种（29个分离株）。然后对这些鉴定的分离株进行了三个基于rDNA位点的系统发育分析。来自25个未知种/分离株的序列也被纳入3个rDNA位点的比较中。首次对麻风属植物的核糖体DNA基因进行了分析，结果表明麻风属植物与丁丁根有密切的亲缘关系，与丁丁科有明显的分支。在19世纪18年代的家谱中出现了丁丁统复合体的4个新分支（6 ~ 9）。ITS和28S区域与18S rDNA的相对变异分析表明，当18S rDNA变异小于3%时，ITS1-5.8S-ITS2和ITS2区域与18S rDNA具有良好的共变性，而当差异小于1%时，比较位点之间不存在相关性。这些发现强调了使用可变的基于位点的切断分歧来限定丁丁属形态种的困难。
To improve understanding of diversity, phylogeny and evolution in tintinnid ciliates, it is essential to link multiple molecular markers with properly identified and documented morphospecies. Accordingly, 54 tintinnid morphospecies/isolates mainly from the Yellow and East China Seas were collected and analysed. Using single-cell approaches, sequences were obtained for three rDNA loci (18S, ITS1-5.8S-ITS2, D1-D5 region of 28S). Twenty-six tintinnid morphospecies (29 isolates) are documented by micrographs, measurements, morphologically described, and compared with the original species description. Three rDNA loci-based phylogenetic analyses were then performed for these identified isolates. Sequences from 25 unidentified species/isolates were also included in the comparison of the three rDNA loci. Ribosomal DNA genes of the genus Leprotintinnus were analysed for the first time, showing that Leprotintinnus was closely related to Tintinnopsis radix and branched distinctly apart from the family Tintinnidiidae. Four novel clades (VI to IX) of the Tintinnopsis complex emerged in the 18S genealogies. Analyses of the relative variability in the ITS and 28S regions vs. the 18S rDNA showed that the ITS1-5.8S-ITS2 and ITS2 regions well co-varied with the 18S rDNA when the variations of the latter were less than 3%, whereas at difference of less than 1%, no correlation was found between the compared loci. These findings highlight the difficulties in using variable locus-based cut-off divergences in circumscribing tintinnid morphospecies.
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DOI：
10.1111/j.1550-7408.2004.tb00567.x
发表时间：
2004-05
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Agatha S;Strüder-Kypke MC;Beran A
通讯作者：
Beran A
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
来自中国沿海水域的三种丁丁纤毛虫：Tintinnopsis tocantinensis、T. radix 和 T. cylindrica（Ciliophora、Spirotrichea）的重新描述
影响因子：
2.9
作者：
Jiang, Yong;Yang, Jinpeng;Lin, Xiaofeng
通讯作者：
Lin, Xiaofeng
影响因子：
4.2
作者：
Bachy, Charles;Moreira, David;Lopez-Garcia, Purificacion
通讯作者：
Lopez-Garcia, Purificacion
影响因子：
5.8
作者：
Huelsenbeck, JP;Ronquist, F
通讯作者：
Ronquist, F