Electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications
Electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications
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DOI：
10.1016/j.ijbiomac.2018.05.099
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发表时间：
2018-09-01
影响因子：
8.2
通讯作者：
Saha, Prosenjit
中科院分区：
文献类型：
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作者：
Chanda, Amit;Adhikari, Jaideep;Saha, Prosenjit
本研究采用毒性较小的溶剂体系制备了由壳聚糖（CS）/聚己内酯（PCL）和透明质酸（HA）组成的机械稳定、生物相容的双层聚合物支架。采用静电纺丝技术制备支架，并对其进行形态学、理化和力学表征。发现CS/PCL-HA双层支架的平均纤维直径为362.2 +/-236 nm，其在细胞外基质中发现的胶原纤维的范围内。与PCL和CS-PCL支架相比，双层支架的溶胀性、降解性、亲水性和水蒸气透过率增强。抗菌性能评价显示双层支架上的细菌粘附减少。vero细胞的体外研究[肾上皮细胞，提取自非洲绿色猴（绿猴属）]证实了Vero细胞在双层CS/PCL-HA支架上的增殖、生长和迁移比在PCL和CS/PCL支架上的增殖、生长和迁移增强。本研究的新奇之处包括使用HA作为机械稳定支架，其生物学特性可接受，可用于伤口愈合应用。(C)2018爱思唯尔B. V.保留所有权利。
Fabrication of mechanically stable, biocompatible bilayered polymeric scaffold consisting of chitosan(CS)/polycaprolactone(PCL) and hyaluronic acid( HA) using less toxic solvent system is presented in this study. Electrospinning technique to make the scaffold was used followed by morphological, physiochemical and mechanical characterizations. Average fiber diameter of CS/PCL-HA bilayered scaffold was found 362.2 +/- 236 nm which is in the range of collagen fiber found in the extracellular matrices. Enhanced swelling, degradation, hydrophilicity and water vapour transmission rate were found for the bilayered scaffold compared to that of the PCL and CS-PCL scaffolds. Antimicrobial property evaluation revealed reduction in bacterial adhesion on bilayered scaffolds. Invitro studies with vero cells [kidney epithelial cell, extracted from African Green Monkey (Chlorocebus sp.)] confirm enhanced proliferation, growth and migration of vero cell on the bilayered CS/PCL-HA scaffold to that of PCL and CS/PCL scaffolds. Novelty of this study includes the use of HA for mechanically stabilized scaffold with acceptable biological properties for wound healing applications. (C) 2018 Elsevier B.V. All rights reserved.