ALTERATION OF IMMUNOREACTIVITY BY HYDRATED AUTOCLAVING, MICROWAVE TREATMENT, AND SIMPLE HEATING OF PARAFFIN-EMBEDDED TISSUE-SECTIONS
ALTERATION OF IMMUNOREACTIVITY BY HYDRATED AUTOCLAVING, MICROWAVE TREATMENT, AND SIMPLE HEATING OF PARAFFIN-EMBEDDED TISSUE-SECTIONS
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DOI：
10.1111/j.1699-0463.1994.tb04879.x
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发表时间：
1994-04-01
期刊：
影响因子：
2.8
通讯作者：
KINO, I
中科院分区：
文献类型：
--
作者：
IGARASHI, H;SUGIMURA, H;KINO, I
研究了水合高压灭菌器（121℃，2atm， 20分钟）、微波炉（水中）和简单加热（60℃蒸馏水过夜或90℃ZnSO4中10分钟）对市购抗体对56种抗原在福尔马林固定石蜡包埋组织切片中染色的影响。核抗原、糖蛋白、淋巴细胞表面标记物和嗜铬粒蛋白A的检出率显著提高，而病毒抗原和肽激素的检出率降低或不变。这种增强不仅包括阳性染色的独特性，而且包括阳性细胞的数量，通过比较系列切片显示。四种加热方式中，微波加热和高压灭菌对p53、c-erbB-2和CA125的效果较好，而对平滑肌肌动蛋白（HHF35和CGA7）的效果最好。一般情况下，加热程序对这些抗原的影响在病例中是一致的，但对GFAP的影响因病例而异。我们观察到的改变可能会显著影响目前流行的肿瘤标志物（如p53）的免疫组织化学染色的解释，其患病率（胃癌为28%对64%；转移性肝癌为36%对82%）和其他重要的诊断标志物。
The effects of treatment in a hydrated autoclave (121 degrees C, 2 atm for 20 min), microwave oven (in water), and simple heating (60 degrees C overnight in distilled water or 90 degrees C for 10 min in ZnSO4) on the stainability of 56 antigens by commercially available antibodies in formalin-fixed paraffin-embedded tissue sections were evaluated. The detectability of nuclear antigens, glycoprotein, lymphocytic surface markers, and chromogranin A was significantly and reproducibly improved by these treatments, whereas the detectability of viral antigens and peptide hormones was attenuated or unchanged. This enhancement includes not only the distinctiveness of the positive staining, but also the number of positive cells, as revealed by comparing serial sections. Among these four heating procedures, microwave heating and autoclaving were more effective than the others on p53, c-erbB-2, and CA125, whereas simple heating was best for smooth-muscle actin (HHF35 and CGA7). Generally the effects of the heating procedures for these antigens were consistent among the cases, but the effects on GFAP varied with the case. The alterations we observed could significantly influence the interpretation of immunohistochemical staining of currently popular tumor markers such as p53 in terms of their prevalence (28% vs 64% in gastric cancer; 36% vs 82% in metastatic liver cancer) and other diagnostically important markers.