Serum and synovial fluid lipidomic profiles predict obesity-associated osteoarthritis, synovitis, and wound repair.
Serum and synovial fluid lipidomic profiles predict obesity-associated osteoarthritis, synovitis, and wound repair.
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DOI：
10.1038/srep44315
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发表时间：
2017-03-20
影响因子：
4.6
通讯作者：
Guilak F
中科院分区：
文献类型：
--
作者：
Wu CL;Kimmerling KA;Little D;Guilak F
高脂肪饮食引起的肥胖是骨关节炎（OA）和伤口愈合减少的主要危险因素。本研究的目的是在临床前肥胖OA小鼠模型中确定血清和滑液脂质水平与OA、滑膜炎、脂肪因子水平和伤口愈合之间的关系。雄性C57BL/6 J小鼠分别饲喂低脂（10% kcal）或富含饱和脂肪酸（sfa）、ω-6或ω-3多不饱和脂肪酸（pufa）的三种高脂（HF, 60% kcal）饲料中的一种。骨关节炎是由内侧半月板失稳引起的。小鼠还接受了耳击，以评估伤口愈合情况。收集血清和滑液进行脂质组学和脂肪因子分析。我们发现血清ω-3 PUFAs水平与OA和伤口大小呈负相关，而与脂联素水平呈正相关。相反，大多数ω-6 PUFAs与OA、愈合受损和炎症脂肪因子呈正相关。有趣的是，五烷基酸（C15:0，一种奇链SFA）和棕榈油酸的水平与关节降解呈负相关。这项研究扩展了我们对FAs与OA、滑膜炎和伤口愈合之间联系的理解，并报道了新发现的血清和滑膜液FAs作为肥胖OA的预测性生物标志物。
High-fat diet-induced obesity is a major risk factor for osteoarthritis (OA) and diminished wound healing. The objective of this study was to determine the associations among serum and synovial fluid lipid levels with OA, synovitis, adipokine levels, and wound healing in a pre-clinical obese mouse model of OA. Male C57BL/6 J mice were fed either a low-fat (10% kcal) or one of three high-fat (HF, 60% kcal) diets rich in saturated fatty acids (SFAs), ω-6 or ω-3 polyunsaturated FAs (PUFAs). OA was induced by destabilization of the medial meniscus. Mice also received an ear punch for evaluating wound healing. Serum and synovial fluid were collected for lipidomic and adipokine analyses. We demonstrated that the serum levels of ω-3 PUFAs were negatively correlated with OA and wound size, but positively correlated with adiponectin levels. In contrast, most ω-6 PUFAs exhibited positive correlations with OA, impaired healing, and inflammatory adipokines. Interestingly, levels of pentadecylic acid (C15:0, an odd-chain SFA) and palmitoleic acid were inversely correlated with joint degradation. This study extends our understanding of the links of FAs with OA, synovitis and wound healing, and reports newly identified serum and synovial fluid FAs as predictive biomarkers of OA in obesity.