Macromolecular uptake of alkyl-chain-modified guanidinoglycoside molecular transporters.
Macromolecular uptake of alkyl-chain-modified guanidinoglycoside molecular transporters.
复制标题
烷基链修饰的胍基糖苷分子转运蛋白的大分子摄取。
DOI：
10.1002/cbic.201300606
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tor,Yitzhak
中科院分区：
文献类型：
--
作者：
Inoue,Makoto;Wexselblatt,Ezequiel;Esko,JeffreyD;Tor,Yitzhak
胍基糖苷类是一种能够递送高分子量生物聚合物的细胞转运蛋白家族，先前已显示对细胞表面硫酸乙酰肝素蛋白聚糖具有高选择性并促进其聚集。在此，通过细胞表面FRET分析研究了两亲性胍基糖苷衍生物的内化机制。出乎意料的是，虽然保持了硫酸乙酰肝素的选择性，但这些衍生物的细胞摄取似乎并不涉及细胞表面上蛋白聚糖的聚集。这表明与母体胍基糖苷类载体相比，存在不同的摄取机制。
Guanidinoglycosides, a family of cellular transporters capable of delivering highMwbiopolymers, have previously been shown to display high selectivity for cell‐surface heparan sulfate proteoglycans and promote their clustering. Herein, the internalization mechanism of amphiphilic guanidinoglycoside derivatives was investigated by cell‐surface FRET analysis. Unexpectedly, although the heparan sulfate selectivity is maintained, the cellular uptake of these derivatives does not appear to involve clustering of the proteoglycans on the cell surface. This suggests a distinct uptake mechanism when compared to the parent guanidinoglycoside‐based carriers.