Immune labeling of certain strains of Actinomyces naeslundii and Actinomyces viscosus by fluorescence and electron microscopy
Immune labeling of certain strains of Actinomyces naeslundii and Actinomyces viscosus by fluorescence and electron microscopy
复制标题
通过荧光和电子显微镜对内氏放线菌和粘性放线菌的某些菌株进行免疫标记
DOI：
10.1128/iai.25.3.1016-1028.1979
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发表时间：
1979
影响因子：
3.1
通讯作者：
M. Listgarten
中科院分区：
文献类型：
--
作者：
C. Lai;M. Listgarten
对12株粘性放线菌（Actinomycesviscosus）和A.在用适当标记的同源和异源菌株的抗血清处理后，通过光学显微镜和透射电子显微镜检查在补充有绵羊红细胞的胰蛋白酶大豆琼脂平板上生长的内氏菌。用同源兔抗血清和异硫氰酸荧光素（FITC）标记的羊抗兔免疫球蛋白G（IgG）孵育细胞，在A. naeslundii和间断的，不规则的荧光轮廓的情况下，人类菌株的A。粘性。不同的标记模式似乎与在超微结构水平上存在的长的、不均匀分布在人A细胞表面的“绒毛”链有关。viscosus细胞，而A.内氏细胞的绒毛层较窄或较均匀。免疫包被反应显示同源抗体与人A表面的不规则绒毛链结合。viscosus细胞的同源抗血清，而A.内氏衣A.具有均匀厚度的适度电子致密抗体包被的内氏细胞。人源A.粘滞细胞与抗A.内氏抗血清和FITC标记的羊抗兔IgG产生的荧光轮廓与同源抗血清不同。A. naeslundii与异源兔抗人A. viscosus菌株和FITC标记的抗兔IgG表现出完全平滑的荧光轮廓，类似于用同源抗血清产生的。A.仓鼠来源的粘性菌株不同于A.通过不存在表面绒毛和通过将这些细胞与同源兔抗血清随后与FITC标记的山羊抗兔IgG孵育产生的相对平滑、均匀的荧光，鉴定人源粘性菌株。抗仓鼠品系的抗血清与A. naeslundii或A.粘性。在本实验的生长条件下，间接荧光技术的超微结构特征和标记模式可能有助于区分这些血清型。
A total of 12 well-characterized strains of Actinomyces viscosus and A. naeslundii grown on Trypticase soy agar plates supplemented with sheep erythrocytes were examined by light microscopy and transmission electron microscopy after treatment with appropriately labeled antisera to homologous and heterologous strains. Cells incubated with homologous rabbit antisera followed by fluorescein-isothiocyanate (FITC)-conjugated goat anti-rabbit immunoglobulin G (IgG) exhibited a completely smooth fluorescent outline in the case of A. naeslundii and and interrupted, irregular fluorescent outline in the case of human strains of A. viscosus. The different labeling patterns appeared to be related to the presence at the ultrastructural level of long, unevenly distributed strands of "fuzz" on the surface of human A. viscosus cells, whereas A. naeslundii cells had a narrower layer of fuzz, or more even thickness. The immunocoating reaction revealed homologous antibody binding to the irregular strands of fuzz on the surface of human A. viscosus cells, whereas homologous antisera to A. naeslundii coated A. naeslundii cells with a moderately electron-dense coating of antibody of even thickness. Human strains of A. viscosus incubated with heterologous antiserum to A. naeslundii followed by FITC-labeled goat anti-rabbit IgG exhibited a segmented fluorescent outline, which differed from that produced with homologous antisera. A. naeslundii incubated with heterologous rabbit antisera to human A. viscosus strains and FITC-labeled anti-rabbit IgG exhibited a completely smooth fluorescent outline similar to that produced with homologous antiserum. A. viscosus strains of hamster origin differed from A. viscosus strains of human origin by the absence of a surface fuzz and the comparatively smooth, even fluorescence produced by incubating these cells with homologous rabbit antiserum followed by FITC-labeled goat anti-rabbit IgG. Antiserum to a hamster strain did not cross-react with A. naeslundii or human strains of A. viscosus. Under the growth conditions of this experiment, ultrastructural features and labeling patterns with the indirect fluorescent technique may be useful in differentiating these serotypes from one another.