ANALYSIS OF AN EVOLUTIONARILY CONSERVED ANTIGENIC SITE ON MAMMALIAN CYTOCHROME-C USING SYNTHETIC PEPTIDES
ANALYSIS OF AN EVOLUTIONARILY CONSERVED ANTIGENIC SITE ON MAMMALIAN CYTOCHROME-C USING SYNTHETIC PEPTIDES
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DOI：
10.1073/pnas.82.5.1508
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
PATERSON, Y
中科院分区：
文献类型：
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作者：
JEMMERSON, R;MORROW, PR;PATERSON, Y
在本研究中使用了两种包含哺乳动物细胞色素c（cyt c）的NH₂末端N -乙酰 -甘氨酸 -天冬氨酸 -缬氨酸 -谷氨酸四肽的合成肽，以解决哺乳动物是否能对一种蛋白质的进化保守区域产生免疫应答这一问题。对这些肽作为免疫原产生抗自身细胞色素c抗血清以及结合兔抗啮齿动物细胞色素c抗体的能力进行了评估。这些研究的结果表明，在细胞色素c的一个包含1 - 4位残基的进化保守区域存在一个免疫原性决定簇。无论是作为载体蛋白上的肽，还是在聚合的哺乳动物细胞色素c的完整分子环境中，这个决定簇都能诱导抗自身细胞色素c抗体。
Two synthetic peptides inclusive of the NH2-terminal N-acetyl-Gly-Asp-Val-Glu tetrapeptide of mammalian cytochrome c (cyt c) were used in this study to address the question of whether mammals can respond immunologically to an evolutionarily conserved region of a protein. These peptides were assessed for their capacity to act as immunogens for the production of anti-self cyt c antisera and to bind rabbit anti-rodent cyt c antibody. The findings from these studies indicate the existence of an immunogenic determinant in an evolutionarily conserved region of cyt c that contains residues 1-4. This determinant can induce anti-self cyt c antibodies whether presented as a peptide on a carrier protein or in the context of the intact molecule as polymerized mammalian cyt c.