Anti-Inflammatory Role of Cannabidiol and O-1602 in Cerulein-Induced Acute Pancreatitis in Mice
Anti-Inflammatory Role of Cannabidiol and O-1602 in Cerulein-Induced Acute Pancreatitis in Mice
复制标题
大麻二酚和 O-1602 在雨蛙素诱导的小鼠急性胰腺炎中的抗炎作用
DOI：
10.1097/mpa.0b013e318259f6f0
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发表时间：
2013-01-01
期刊：
影响因子：
2.9
通讯作者：
Storr, Martin
中科院分区：
文献类型：
--
作者：
Li, Kun;Feng, Jia-yan;Storr, Martin
目的：本实验观察了G蛋白偶联受体55（GPR 55）配体O-1602和大麻二酚（CBD）对实验性急性胰腺炎（AP）的抗炎作用。在第一次注射前30分钟和第五次注射雨蛙肽前立即通过腹膜内注射给予药物（O-1602，10 mg/kg，或CBD，0.5 mg/kg）2次。末次注射后3 h，取血、肺、胰腺，测定胰腺酶活性、髓过氧化物酶活性、促炎细胞因子活性，并检测胰腺GPR 55 mRNA和蛋白的表达。Cannabidiol或O-1602治疗显著改善AP小鼠的病理变化，降低酶活性，IL-6和肿瘤坏死因子α水平，以及血浆和器官组织中的髓过氧化物酶活性。结论：大麻二酚和O-1602对AP小鼠具有抗炎作用，并能提高胰腺组织GPR 55的表达。
Objectives: The anti-inflammatory effects of O-1602 and cannabidiol (CBD), the ligands of G protein-coupled receptor 55 (GPR55), on experimental acute pancreatitis (AP) were investigated.Methods: Acute pancreatitis was induced in C57BL mice by intraperitoneal injection of 50 mu g/kg cerulein hourly, with a total of 6 times. Drugs (O-1602, 10 mg/kg, or CBD, 0.5 mg/kg) were given by intraperitoneal injection 2 times at 30 minutes before the first injection and immediately before the fifth cerulein injection. At 3 hours after the last injection, the blood, the lungs, and the pancreas were harvested for the pancreatic enzyme activity, myeloperoxidase activity, and pro-inflammatory cytokines measurement; and the expressions of GPR55 mRNA and protein in the pancreas were detected.Results: Cannabidiol or O-1602 treatment significantly improved the pathological changes of mice with AP and decreased the enzyme activities, IL-6 and tumor necrosis factor alpha levels, and the myeloperoxidase activities in plasma and in the organ tissues. G protein-coupled receptor 55 mRNA and protein expressed in the pancreatic tissue, and the expressions were decreased in the mice with AP, and either CBD or O-1602 attenuated these changes to a certain extent.Conclusion: Cannabidiol and O-1602 showed anti-inflammatory effects in mice with AP and improved the expression of GPR55 in the pancreatic tissue as well.