An easy method to identify 8-keto-15-hydroxytrichothecenes by thin-layer chromatography
An easy method to identify 8-keto-15-hydroxytrichothecenes by thin-layer chromatography
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薄层色谱法鉴定 8-酮-15-羟基单端孢霉烯族化合物的简便方法
DOI：
10.2520/myco.58.115
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Kimura
中科院分区：
文献类型：
--
作者：
Naoko;Takahashi;Ando;Takeshi;Tokai;Minoru;Yoshida;Makoto;Fujimura;Kimura
4-（对硝基苯）吡啶（NBP）常用于薄层色谱（TLC）板上显影的毛霉烯的检测，因为这种显色试剂可以特异性检测毛霉烯的12,13-环氧基团。与NBP热反应，室温冷却几分钟后，TLC板上的trichoccenes用四乙基苯胺（TEPA）碱化，可见为蓝色斑点。当薄层色谱板放置过夜，然后喷上TEPA，大多数毛霉烯产生类似的蓝色。8-酮-15-羟基毛菌素则呈暗黄绿色斑点。这种改进的检测方法可以作为其他仪器分析的简单替代方法，用于区分8-酮-15-羟基毛霉烯和其他在TLC板上给出相似R f值的毛霉烯。
4-( p -nitrobenzyl)pyridine (NBP) is often used for detection of trichothecenes developed on a thin-layer chromatography (TLC) plate, because this chromogenic reagent enables specific detection of the 12,13-epoxide group of trichothecenes. After the heat reaction with NBP and cooling at room temperature for several minutes, trichothecenes on the TLC plate are visualized as blue spots by alkalization with tetraethylenepentamine (TEPA). When the TLC plate was left overnight and then sprayed with TEPA, most trichothecenes yielded similar blue colors. However, dark yellowish green spots were given for 8-keto-15-hydroxytrichothece-nes. This modified detection method can be used as an easy alternative of other instrumental analyses to distinguish between 8-keto-15-hydroxytrichothecenes and other trichothecenes that give similar R f values on a TLC plate.