Correlation of chondroitin sulfate proteoglycan expression on proliferating brain capillary endothelial cells with the malignant phenotype of astroglial cells.
Correlation of chondroitin sulfate proteoglycan expression on proliferating brain capillary endothelial cells with the malignant phenotype of astroglial cells.
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发表时间：
1991-09
期刊：
影响因子：
11.2
通讯作者：
M. Schrappe;F. Klier;R. Spiro;T. Waltz;R. Reisfeld;C. Gladson
中科院分区：
文献类型：
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作者：
M. Schrappe;F. Klier;R. Spiro;T. Waltz;R. Reisfeld;C. Gladson
人胶质母细胞瘤（5/5）是最恶性的星形胶质细胞源性肿瘤，特异性表达硫酸软骨素蛋白聚糖，该蛋白聚糖被单克隆抗体9.2.27识别并定位于胶质瘤细胞表面、增殖内皮细胞和肿瘤床内的血管周围细胞外基质。相反，这种蛋白聚糖在正常成人新皮质和白色物质中的表达仅限于小动脉的平滑肌，而正常胶质细胞、内皮细胞和内皮细胞基底膜是无反应的。此外，两个间变性星形细胞瘤，代表中等级别的星形胶质细胞源性肿瘤，未能与单克隆抗体9.2.27反应。在培养中，胶质母细胞瘤和毛细血管脑内皮细胞特异性合成250 kDa的核心蛋白和高分子量硫酸软骨素蛋白聚糖，由单克隆抗体9.2.27识别。这些数据表明，这种硫酸软骨素蛋白多糖的表达之间的相关性，对增殖的脑毛细血管内皮细胞和星形胶质细胞的恶性表型。硫酸软骨素蛋白聚糖的突出血管周围定位使其成为增殖的脑毛细血管内皮细胞和最恶性转化的星形胶质细胞的标志物，从而为胶质母细胞瘤的免疫治疗提供了理想的靶点。
Human glioblastomas (five of five), the most malignant astroglial-derived tumors, specifically express a chondroitin sulfate proteoglycan that is recognized by monoclonal antibody 9.2.27 and localized to the glioma cell surface, proliferating endothelial cells, and the perivascular extracellular matrix within the tumor bed. In contrast, the expression of this proteoglycan in normal adult neocortex and white matter is limited to the smooth muscle of small arteries, while normal glia, endothelial cells, and endothelial cell basement membranes are nonreactive. Moreover, two anaplastic astrocytomas, representing medium-grade astroglial-derived tumors, fail to react with monoclonal antibody 9.2.27. In culture, glioblastoma and capillary brain endothelial cells specifically synthesize a 250-kDa core protein and a high-molecular-mass chondroitin sulfate proteoglycan, recognized by monoclonal antibody 9.2.27. These data suggest a correlation between the expression of this chondroitin sulfate proteoglycan on proliferating brain capillary endothelial cells and the malignant phenotype of astroglial cells. The prominent perivascular localization of chondroitin sulfate proteoglycan makes it a marker for both proliferating brain capillary endothelial cells and the most malignant transformed astroglial cells, thus providing an ideal target for the immunotherapy of glioblastoma.