Quantitative experimental study of shear stresses and mixing in progressive flow regimes within annular-flow bioreactors
Quantitative experimental study of shear stresses and mixing in progressive flow regimes within annular-flow bioreactors
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环流生物反应器内渐进流态剪切应力和混合的定量实验研究
DOI：
10.1016/j.ces.2004.07.044
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发表时间：
2004
影响因子：
4.7
通讯作者：
Richard A. Black
中科院分区：
文献类型：
--
作者：
S. Curran;Richard A. Black
环流生物反应器通常在层流库埃特流条件下运行，以尽量减少剪切引起的细胞损伤。在这项研究中，我们使用自由悬浮粒子的剪切速率模型，结合二维速度场的激光多普勒风速测量数据，在两个几何尺度上，计算了从Couette流到Taylor-vortex流的层流状态下模型环状血管中的流体剪切应力。在两种情况下，各壁面附近的边界层剪切应力最大，其值通常是环空空间平均应力的6倍；然而，在研究的两个血管中，较大的血管中它们各自的幅度明显较低。细胞活力研究也进行了，其中哺乳动物细胞在动态条件下培养在一个功能生物反应器具有相同的尺寸较小的容器。这些研究结果表明，在Taylor-vortex流动中，悬浮细胞的数量明显多于在Couette流动中，但以更高Taylor数的细胞活力为代价。综上所述，这些发现表明，在动态相似的流动条件下，在较大的血管中，Taylor-Couette流所提供的增强对流质量传输的好处可以在没有明显的剪切损伤细胞和组织的风险的情况下实现。
Annular-flow bioreactors are normally operated under laminar Couette flow conditions in order to minimise shear-induced damage to cells. In this study, we computed the fluid shear stresses in model annular vessels over a range of laminar flow regimes, from Couette flow to Taylor-vortex flow, and at two geometric scales, using a shear rate model for freely suspended particles, together with experimental Laser Doppler Anemometry data for a 2-D velocity field. The shear stresses were greatest in the boundary layers adjacent to each wall in each case, with values typically 6 times higher than the mean stresses in the annular space; their respective magnitudes were significantly lower in the larger of the two vessels studied, however. Cell viability studies were also performed in which mammalian cells were cultured under dynamic conditions in a functional bioreactor having the same dimensions as the smaller vessel. The results of these studies demonstrated that a significantly greater number of cells remained in suspension in Taylor-vortex flows than in Couette flow, but at the expense of cell viability at higher Taylor numbers. Taken together, these findings suggest that the benefits of enhanced convective mass transport afforded by Taylor–Couette flows could be realised without risk of appreciable shear induced damage of cells and tissues in larger vessels operating under dynamically similar flow conditions.
具有流体流动的三维培养中的人成骨细胞样细胞。
DOI：
--
发表时间：
2003
期刊：
Biorheology.
影响因子：
--
作者：
Botchwey,EdwardA;Pollack,SolomonR;El-Amin,Saadiq;Levine,ElliotM;Tuan,RockyS;Laurencin,CatoT
通讯作者：
Laurencin,CatoT