Resistance status of Anopheles gambiae (s.l.) to four commonly used insecticides for malaria vector control in South-East Nigeria
Resistance status of Anopheles gambiae (s.l.) to four commonly used insecticides for malaria vector control in South-East Nigeria
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DOI：
10.1186/s13071-020-04027-z
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发表时间：
2020-03-24
影响因子：
3.2
通讯作者：
Gnanguenon, Virgile
中科院分区：
文献类型：
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作者：
Chukwuekezie, Okechukwu;Nwosu, Emmanuel;Gnanguenon, Virgile
背景全球疟疾病媒控制取得的进展主要归功于杀虫剂的使用。然而，由于包括杀虫剂抗药性在内的一系列因素，防治疟疾的成功已经放缓甚至停滞。非洲，特别是尼日利亚感受到了这一疾病的最大负担。不幸的是，该国许多地区，特别是东南区，缺乏关于杀虫剂抗药性的充分信息。因此，这项研究的目的是弥合信息差距，在区。MethodsThe研究进行了四月至十二月2016年。冈比亚按蚊（s.l.）从东南区五个州的一个社区收集幼虫和蛹，并饲养至成虫阶段。根据世界卫生组织的测试程序，对成年人进行了杀虫剂抗性的生物测定，涉及公共卫生中使用的四类杀虫剂。结果100%的蚊虫对苯虫威敏感，但对甲基抗蚜胺（39.6%）、溴氰菊酯（57%）和滴滴涕（13%）耐药。分子生物学分析表明，只有一个。冈比亚按蚊（严格意义上的冈比亚按蚊）在除埃邦伊以外的所有州都有发现，埃邦伊只有科卢兹按蚊。在该区域发现了高频率（0.6-0.9）的L1014 F突变。L1014 F突变在An中显著较高。冈比亚（s.s.）比在安。coluzzii（P
BackgroundProgress made in the control of malaria vectors globally is largely due to the use of insecticides. However, success in the fight against malaria has slowed down or even stalled due to a host of factors including insecticide resistance. The greatest burden of the disease is felt in Africa, particularly Nigeria. Unfortunately, adequate information on insecticide resistance is lacking in many parts of the country, particularly the South-East Zone. Hence, this study aims to bridge the information gap in the Zone.MethodsThe study was conducted from April to December 2016. Anopheles gambiae (s.l.) larvae and pupae were collected from one community each, in the five states of the South-East Zone and reared to the adult stage. The adults were subjected to bioassays for insecticide resistance in accordance with the World Health Organization test procedures, across the four classes of insecticides used in public health. The mosquitoes were also subjected to molecular identification to the species level, and genotyped for West African knockdown resistance mutation (L1014F) and insensitive acetylcholinesterase-1 resistance mutation (G119S).ResultsThe mosquitoes were susceptible (100%) to bendiocarb but resistant to pirimiphos-methyl (39.6%), deltamethrin (57%) and dichlorodiphenyltrichloroethane (DDT) (13%). Molecular analysis revealed that only An. gambiae (sensu stricto) was found in all the states except for Ebonyi, where only Anopheles coluzzii was present. High frequencies (0.6-0.9) of the L1014F mutation were found across the zone. The L1014F mutation was significantly higher in An. gambiae (s.s.) than in An. coluzzii (P