Extracellular matrix assembly stress drives Drosophila central nervous system morphogenesis
Extracellular matrix assembly stress drives Drosophila central nervous system morphogenesis
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细胞外基质组装应激驱动果蝇中枢神经系统形态发生
DOI：
10.1101/2022.04.18.488510
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Serna-Morales E
中科院分区：
文献类型：
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作者：
Serna-Morales E
控制组织形态发生的力量归因于细胞驱动的活动，细胞外基质（ECM）的任何作用都被认为是被动的。然而，所有聚合物网络，包括ECM，理论上可以在其组装过程中产生自主应力。在此，我们通过分析果蝇腹侧神经索（VNC）凝聚过程中的新生ECM组装，研究ECM在达到稳态平衡之前的形态发生功能。不对称的VNC缩短和表面积的快速减少与组织周围的胶原蛋白-IV（Col 4）的指数组装相关。同时，短暂的发育诱导的Col 4梯度导致ECM的连贯长程流动，这平衡了Col 4网络。有限元分析和扰动的Col 4网络的形成，通过产生占主导地位的Col 4截断，影响组装，揭示了VNC形态动力学是由ECM驱动的表面张力突然增加。这些数据强调了ECM组装应力和相关的网络不稳定性可以积极参与组织形态发生。
The forces controlling tissue morphogenesis are attributed to cellular-driven activities and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can theoretically develop autonomous stresses during their assembly. Here we examine the morphogenetic function of an ECM prior to reaching homeostatic equilibrium by analyzingde novoECM assembly duringDrosophilaventral nerve cord (VNC) condensation. Asymmetric VNC shortening and a rapid decrease in surface area correlate with exponential assembly of Collagen-IV (Col4) surrounding the tissue. Concomitantly, a transient developmentally-induced Col4 gradient leads to coherent long-range flow of ECM, which equilibrates the Col4 network. Finite element analysis and perturbation of Col4 network formation through the generation of dominant Col4-truncations that affect assembly, reveals that VNC morphodynamics is driven by a sudden increase in ECM-driven surface tension. These data highlight that ECM assembly stress and associated network instabilities can actively participate in tissue morphogenesis.