Mechanisms for how inhaled multiwalled carbon nanotubes suppress systemic immune function in mice.
Mechanisms for how inhaled multiwalled carbon nanotubes suppress systemic immune function in mice.
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吸入多壁碳纳米管如何抑制小鼠全身免疫功能的机制。
DOI：
10.1038/nnano.2009.151
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发表时间：
2009-07
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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作者：
吸入碳纳米管对健康的潜在影响很重要，因为可能会在职业环境中接触碳纳米管。此前，我们发现吸入多壁碳纳米管（MWCNT）的小鼠表现出全身免疫功能受到抑制。在这里，我们展示了这种免疫抑制的机制。小鼠在全身吸入室中连续 14 天暴露于 0、0.3 或 1 mg/m3 MWCNT 中，每天 6 小时。那些暴露于 1 mg/m3 的人表现出全身免疫功能受损。暴露的动物的脾细胞增加了前列腺素合酶的基因表达，并在用布洛芬治疗时从免疫抑制中解救出来。 Cyclooxygenase-2 基因敲除小鼠对 MWCNT 诱导的抑制具有抵抗力。从暴露小鼠肺部分离的蛋白质含有转化生长因子-β，它会抑制野生型脾细胞的免疫功能，但不会抑制体外敲除小鼠的免疫功能。这表明来自肺部的信号可以激活脾脏中的信号，从而抑制暴露小鼠的免疫功能。
The potential health effects of inhaling carbon nanotubes are important because of possible exposures in an occupational setting. Previously, we showed that mice inhaling multiwalled carbon nanotubes (MWCNT) showed suppressed systemic immune function. Here we show the mechanisms for this immune suppression. Mice were exposed to 0, 0.3, or 1 mg/m3 MWCNT for 6h/day for 14 consecutive days in whole-body inhalation chambers. Those exposed to 1 mg/m3 showed compromised systemic immune function. Spleen cells from exposed animals increased gene expression of prostaglandin synthase enzymes and were rescued from immunosuppression when treated with ibuprofen. Cyclooxygenase-2 knockout mice were resistant to MWCNT-induced suppression. Proteins isolated from the lungs of exposed mice contained transforming growth factor-beta, which suppressed immune function of wild-type splenocytes but not those from knockout mice in vitro. This suggests that signals from the lung can activate signals in the spleen to suppress the immune function of exposed mice.