Methylquercetins stimulate melanin biosynthesis in a three-dimensinal skin model.
Methylquercetins stimulate melanin biosynthesis in a three-dimensinal skin model.
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甲基槲皮素在三维皮肤模型中刺激黑色素生物合成。
DOI：
10.1007/s11418-018-1175-0
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发表时间：
2018
影响因子：
3.3
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Yamauchi;K.;Mitsunaga;T.
在之前的研究中，我们发现合成的3- o -甲基槲皮素（3MQ）和3,4 ‘，7- o -三甲基槲皮素（34 ’ 7TMQ）都增加了细胞外黑色素含量。34′7TMQ通过刺激p38通路和小眼相关转录因子（MITF）的表达增加黑素生成酶的活性。相反，在没有MITF参与的情况下，3MQ增加了黑素生成酶的活性，这表明3MQ抑制了黑素生成酶的降解。在本研究中，我们利用商业三维（3D）皮肤模型系统研究了3MQ和34 ' 7TMQ对正常人类黑素细胞黑色素生成的影响。3MQ和34’7TMQ均能延长来自高加索人供体的正常人类黑素细胞的树突，但不刺激黑素细胞的黑色素生成。在包含来自亚洲供体的黑色素细胞的3D皮肤模型中，3MQ和34 ' 7TMQ增加并延长了黑色素细胞，并显示出刺激黑色素生成的倾向。这些结果表明，3MQ和374tmq可以在护肤产品和预防头发变白的药物中得到实际应用。
In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4′,7-O-trimethylquercetin (34′7TMQ) increased extracellular melanin content. 34′7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF). In contrast, 3MQ increased the activity of melanogenic enzymes without the involvement of MITF, which suggests that 3MQ inhibits the degradation of melanogenic enzymes. In the present study, we investigated the effects of 3MQ and 34′7TMQ on melanogenesis in normal human melanocytes and using a commercial three-dimensional (3D) skin model system. Both 3MQ and 34′7TMQ elongated the dendrites of normal human melanocytes from a Caucasian donor, but did not stimulate melanogenesis in the melanocytes. In the 3D skin model, which included melanocytes from an Asian donor, 3MQ and 34′7TMQ increased and elongated the melanocytes and showed a tendency to stimulate melanogenesis. These results suggest that 3MQ and 34′7TMQ could be put to practical use in skin care products and agents aimed at preventing hair graying.