Interactions in biofilms between Listeria monocytogenes and resident microorganisms from food industry premises
Interactions in biofilms between Listeria monocytogenes and resident microorganisms from food industry premises
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DOI：
10.1016/j.ijfoodmicro.2004.03.031
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发表时间：
2004-12-15
影响因子：
5.4
通讯作者：
Chassaing, D
中科院分区：
文献类型：
--
作者：
Carpentier, B;Chassaing, D
将29株细菌与单核增生李斯特菌一起培养成二元生物膜，考察其对李斯特菌在不锈钢表面沉降的影响。大多数菌株经清洗和消毒后从食品加工厂分离，并通过APILAB Plus 3.3.3数据库（bioMerieux）初步鉴定。其中16种降低了单核增生乳杆菌生物膜菌落形成单位（CFU）计数。三种菌株，芽孢杆菌sp. ccl9，一种未确定的革兰氏阳性菌株ccl59和荧光假单胞菌E9.1，导致单纯的单核增生乳杆菌生物膜和混合生物膜之间的CFU计数差异为3对数。11株菌株无影响，只有变种葡萄球菌CCL 73、头型葡萄球菌CCL 54、嗜酒寡养单胞菌CCL 47和睾酮单胞菌CCL 24有阳性影响，单核增生乳杆菌生物膜CFU计数增加0.5 ~ 1.0 log。单增乳杆菌以单细胞形式存在，但在二元生物膜中，单增乳杆菌的空间排列方式不同：(1)在变异乳杆菌CCL 73、变异乳杆菌CCL 56和冠状乳杆菌CCL 54中，单增乳杆菌的细胞聚集在伴侣菌株的微菌落周围；（ii）在睾酮C. CCL 24和CCL 25这两种革兰氏阴性菌株的作用下，单核增生L.细胞形成了自己的微菌落。菌株在纯培养生物膜中的胞外多糖生产能力与其对混合生物膜中单核增生乳杆菌数量的影响之间没有联系。在C. testosterone CCL 24菌株中，从纯培养生物膜中加入经过过滤器消毒的上清液到纯培养的单核增生乳杆菌中，增加了单核增生乳杆菌粘附在不锈钢板上并形成微菌落的数量。这项研究表明，“室内菌群”对在惰性表面上发现单核细胞增生乳杆菌的可能性有很强的影响。(C) 2004 Elsevier B.V.版权所有
Twenty nine bacterial strains were grown as binary culture biofilms with Listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. Most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the APILAB Plus 3.3.3 database (bioMerieux). Sixteen of them decreased L. monocytogenes biofilm colony forming units (CFU) counts. Three strains, Bacillus sp. CCL 9, an unidentified Gram-positive strain CCL 59 and Pseudomonas fluorescens E9.1, led to a 3-log difference in CFU counts between the pure L. monocytogenes biofilms and, the mixed biofilms. Eleven strains had no effect and only four, Kocuria varians CCL 73, Staphylococcus capitis CCL 54, Stenotrophomonas maltophilia CCL 47 and Comamonas testosteroni CCL 24, had a positive effect, with a 0.5- to 1.0-log increase in the L. monocytogenes biofilm CFU counts. On its own, L. monocytogenes settled as single cells, but in binary biofilms, different spatial arrangements were observed: (i) with K. varians CCL 73, K. varians CCL 56 and S. capitis CCL 54, L. monocytogenes cells gathered around the microcolonies of the partner strain; (ii) with the two Gram-negative strains, C. testosteroni CCL 24 and CCL 25, L. monocytogenes cells formed its own microcolonies. No link could be found between the exopolysaccharide production capacity of the bacterial strains in pure-culture biofilms and their effect on the L. monocytogenes population in mixed biofilms. With one strain, C. testosteroni CCL 24, adding filter-sterilized supernatant from a pure-culture biofilm to a pure culture of L. monocytogenes increased the number of L. monocytogenes cells adhering to the stainless steel coupons and forming microcolonies. This study suggests that the "house flora" can have a strong effect on the likelihood of finding L. monocytogenes on inert surfaces. (C) 2004 Elsevier B.V. All rights reserved.