CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda
CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda
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CRISPR/Cas9介导的突变揭示Pax6对于十足目Exopalaemon carinicauda复眼的发育至关重要
DOI：
10.1016/j.ydbio.2020.07.001
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发表时间：
2020-09-15
影响因子：
2.7
通讯作者：
Li，Fuhua
中科院分区：
文献类型：
--
作者：
Gao，Yi;Zhang，Xiaoxi;Li，Fuhua
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The compound eye in crustaceans is a main eye type in the animal kingdom, knowledge about the mechanism to determine the development of compound eye is very limited.Paired box protein 6(Pax6) is generally regarded as a master regulator for eye development. In the present study, a genome-based analysis of thePax6gene in the ridge tail white prawnExopalaemon carinicaudawas performed and two members ofPax6homologs, namedEc-Eyeless(EcEy) andEc-Twin of eyeless(EcToy) were identified. To understand the function of these two homologs ofPax6gene in the prawn, the CRISPR/Cas9 genome editing technique was applied to generateEcEyandEcToyknock-out (KO) prawns and their phenotypes were analyzed. The survivingEcEy-KO embryos and larvae exhibited severe abnormal eye morphology, suggesting thatEcEyis necessary for the compound eye development in prawn, while no mutant phenotype was found inEcToy-KO individuals. These findings highlighted the conservative role ofPax6gene in the compound eye formation, and the functional differentiation betweenEcEyandEcToygene may reveal a novel regulating mechanism ofPax6on the compound eye development in the decapods. These data will provide important information for understanding the regulation mechanism for crustacean compound eye development.