Response of interleukin-1beta in the magnocellular system to salt-loading.
Response of interleukin-1beta in the magnocellular system to salt-loading.
复制标题
大细胞系统中白介素-1β 对盐负荷的反应。
DOI：
10.1111/j.1365-2826.2006.01490.x
复制
发表时间：
2006
影响因子：
3.2
通讯作者：
Phillips,TM
中科院分区：
文献类型：
--
作者：
Summy-Long,JY;Hu,S;Pruss,A;Chen,X;Phillips,TM
饮用 2% NaCl 会降低神经叶中的白细胞介素 (IL)-1β，并增强大细胞神经元和垂体细胞中 IL-1 1 型受体的表达。为了量化渐进盐负荷过程中神经叶细胞因子的消耗，并确定这些变化是否可逆以及是否与加压素 (VP) 或催产素 (OT) 的储存相对应，大鼠在第 0 天喝水，然后喝 2% 氯化钠，持续 2、5、8 或 5 天，然后喝 5 天水（补液）。只喝水的对照大鼠与 5 天加盐的动物配对喂养。第8天将动物斩首，冷冻神经叶并通过放射免疫测定（OT、VP）或酶联免疫吸附测定（IL-1β）分析血浆激素。通过免疫毛细管电泳和激光诱导荧光检测对神经叶匀浆中的 IL-1β、VP 和 OT 进行定量。通过anova、Tukey t 检验、Dunnett 程序、Fisher 最小显着差异和线性回归分析确定差异。作为对盐负荷的反应，大鼠的体重减轻与配对喂养对照组相似，逐渐饮用更多的 2% NaCl，并排出更多的尿液。血浆 VP 在盐负荷第 2 天和第 8 天增加，而渗透压、OT 和细胞因子在 8 天后增加，补液后 IL-1β 仍然升高。在神经叶中，从第 2 天（IL-1β；VP）或第 5 天（OT）开始，随着盐负荷持续时间的增加（IL-1β，r2= 0.98；OT，r2= 0.94；VP，r2= 0.93），所有三种肽逐渐减少，仅通过补液补充 VP。 IL-1β 随 OT (r2= 0.96) 下降的幅度比 VP (r2= 0.86) 更接近（P<0.0001；方差分析），表明慢性脱水过程中神经叶的共同释放。 IL-1β 对神经叶大细胞末梢、垂体细胞和小胶质细胞的局部影响，通过循环细胞因子激活前脑渗透调节结构，可能在长期盐负荷过程中维持 OT 和 VP 的神经分泌。
Drinking 2% NaCl decreases interleukin (IL)‐1β in the neural lobe and enhances IL‐1 Type 1 receptor expression in magnocellular neurones and pituicytes. To quantify cytokine depletion from the neural lobe during progressive salt loading and determine whether the changes are reversible and correspond with stores of vasopressin (VP) or oxytocin (OT), rats were given water on day 0 and then 2% NaCl to drink for 2, 5, 8 or 5 days followed by 5 days of water (rehydration). Control rats drinking only water were pair‐fed amounts eaten by 5‐day salt‐loaded animals. Animals were decapitated on day 8, the neural lobe frozen and plasma hormones analysed by radioimmunoassay (OT, VP) or enzyme‐linked immunosorbent assay (IL‐1β). IL‐1β, VP and OT in homogenates of the neural lobe were quantified by immunocapillary electrophoresis with laser‐induced fluorescence detection. Differences were determined byanova, Tukey's t‐test, Dunnett's procedure, Fisher's least significant difference and linear regression analysis. In response to salt‐loading, rats lost body weight similar to pair‐fed controls, drank progressively more 2% NaCl and excreted greater urine volumes. Plasma VP increased at days 2 and 8 of salt‐loading, whereas osmolality, OT and cytokine were enhanced after 8 days with IL‐1β remaining elevated after rehydration. In the neural lobe, all three peptides decreased progressively with increasing duration of salt‐loading (IL‐1β, r2= 0.98; OT, r2= 0.94; VP, r2= 0.93), beginning on day 2 (IL‐1β; VP) or 5 (OT), with only VP replenished by rehydration. IL‐1β declined more closely (P<0.0001;anovainteraction analysis) with OT (r2= 0.96) than VP (r2= 0.86), indicative of corelease from the neural lobe during chronic dehydration. Local effects of IL‐1β on magnocellular terminals, pituicytes and microglia in the neural lobe with activation of forebrain osmoregulatory structures by circulating cytokine may sustain neurosecretion of OT and VP during prolonged salt‐loading.