The effect of insulin on plasma-membrane and mitochondrial-membrane potentials in isolated fat-cells.
The effect of insulin on plasma-membrane and mitochondrial-membrane potentials in isolated fat-cells.
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胰岛素对分离脂肪细胞质膜和线粒体膜电位的影响。
DOI：
10.1042/bj1960133
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
B. Martin
中科院分区：
文献类型：
--
作者：
R. Davis;M. Brand;B. Martin
1.最近发展起来的利用~(86)Rb~+和[~H]甲基三苯基膦~+的分布来测量完整细胞的质膜和线粒体膜电位的技术，使我们能够表征一种新的胰岛素对脂肪细胞线粒体的作用。对照组细胞质膜和线粒体膜电位分别为75 mV和152 mV。胰岛素(10mU/ml)使细胞质膜超极化9 mV，线粒体膜去极化19 mV。2.在生理胰岛素浓度(10mU/ml)下观察到胰岛素依赖的线粒体去极化，当细胞代谢多种底物时表现出明显的去极化。3.脂肪细胞摄取弱酸5，5-二甲基恶唑烷-2，4-二酮的证据表明，胰岛素使线粒体的pH梯度增大。4.胰岛素改变了构成线粒体质子动力的电和pH梯度成分之间的平衡。提出了一个模型。
1. A recently developed technique for the measurement of plasma-membrane and mitochondrial-membrane potentials in intact cells by using the distribution of 86Rb+ and [3H]methyltriphenylphosphonium+ has enabled us to characterize a novel insulin effect on fat-cell mitochondria. For control cells the plasma-membrane and mitochondrial-membrane potentials were 75 mV and 152 mV respectively. Insulin (10 mu units/ml) caused a 9 mV hyperpolarization of the plasma membrane and a 19 mV depolarization of the mitochondrial membrane. 2. The insulin-dependent mitochondrial depolarization was observed at physiological insulin concentrations (10 mu units/ml) and was apparent when the cells metabolized a wide variety of substrates. 3. Evidence from the uptake of the weak acid 5,5-dimethyloxazolidine-2,4-dione by fat-cells was interpreted as indicating that the mitochondrial pH gradient was increased by insulin. 4. Insulin alters the balance between the electrical and pH-gradient components that form the mitochondrial protonmotive force. A model is proposed.